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Superoxide Dismutase (SOD) Cell Technology Oxidative Stress Detection
SOD
Superoxide Dismutase (SOD) Detection Kit ™: Colorimetric Detection Kit

$225.00

in stock
Product code: CSOD100
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Product Description

Key Benefits

  • 100% Inhibition by Super Oxide Dismutase (SOD).
  • Can detect low concentrations of SOD.
  • Highly water-soluble formazan dye.
  • Applications: Colorimetric detection.

Assay Principle

Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.

sod1

Figure 1. Inhibition Curve Prepated Using SOD from Bovine Liver.

sod2

Figure 2. SOD ASSAY Reaction

Citations

Photocatalytic disinfection of marine bacteria using fluorescent light - T.Y Leung, C. Y Chan,C. Hu, J.C Yu and p.k. wong - Water Research vol 42 issue 19 - December 2008 pp 4827-4837

Vascular oxidative stress and nitric oxide depletion in HIV-1 transgenic rats are reversed by glutathione Restoration - Erik R. Kline, Dean J. Kleinhenz, Bill Liang, Sergey Dikalov, David M. Guidot, C. Michael Hart, Dean P. Jones, and Roy L. Sutliff - AJP - Heart June 2008 vol. 294 no. 6, H2792-H2804

References

  • Malstrom, B., Andreasson, L., and Reinhammer, B. in The Enzymes. Byer, P., editor. XIIB, Academic Press, New York (1975).
  • Lontz, W., Sirsjo, J., Liu, W., Lindberg, M., Rollman, O., and G. (1976) Int. J. Cancer 17, 62–70. Torma, H. (1995) Free Radical Biol. Med. 18, 349–355.
  • Janero, D. R. (1995) CRC Crit. Revs. Food Sci. Nutr. 35, 65–81
  • Dominguez-Rodriguez, J.R. et al. (2001) Anticancer Res. 21:1869.
  • Zhao, Y. et al. (2001) Antioxid. Redox Signal 3:375.
  • # H. Ukeda, A. K. Sarker, D. Kawana and M. Sawamura, Anal. Sci., 15, 353 (1999).

Kit contents and Long Term storage

ReagentPart#Temperature
20X WST-1 Solution, 1 mlPart # 40142-8°C
Xanthine Oxidase Solution (XO), 20uLPart # 60102-8°C
Assay Buffer, 20 mLPart # 30292-8°C
Xanthine Oxidase Dilution Buffer, 10mL. (XO Dilution Buffer)Part # 30302-8°C
SOD Enzyme, 30uL. See vial for acivityPart # 60112-8°C
96 Well ELISA Plate, 1 platePart # 90012-8°C
Adhesive Plate Cover, Qty. 2Part # 90022-8°C
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