- Additional information
- ASSAY PRINCIPLE
- Readable Documents
- Kit contents and storage
Superoxide dismutase (SOD) are metalloenzymes that catalyze the dismutation of superoxide radical into hydrogen peroxide (H2O2) + molecular oxygen (O2) and consequently provide an important defense mechanism against superoxide radical toxicity (1). Oxidative stress dependent upon superoxide radical can account for a number of acute and chronic disease states, which include inflammation and ischemia-reperfusion (2,3). SOD protects murine peritoneal macrophages from apoptosis induces by adriamycine (4). Furthermore, over expression of SOD in fibrosarcome cells, protects against apoptosis and promotes cell differentiation (5).
- 100% Inhibition by Super Oxide Dismutase (SOD).
- Can detect low concentrations of SOD.
- Highly water-soluble formazan dye.
- Applications: Colorimetric detection.
Cell Technology’s SOD kit utilizes a water-soluble tetrazolium salt (WST-1) that produces a highly water-soluble formazan dye upon reduction with a superoxide anion (6). The rate of the reduction with O2.- is linearly related to the xanthine oxidase (XO) activity, and is inhibited by SOD, as shown in Figure 1. Therefore, the IC 50 (50% inhibition activity of SOD or SOD-like materials) can be determined by colorimetric method. Absorbance can be measured at 440nm.
Other direct and indirect methods have been developed but have issues with poor water solubility of the formazan dye and the interaction with the reduced form of xanthine oxidase. Cytochrome C is also commonly used for SOD activity detection, however, its reactivity with superoxide is too high to determine low levels of SOD activity.
Figure 1. Inhibition Curve Prepated Using SOD from Bovine Liver.
Figure 2. SOD ASSAY Reaction
Kit contents and storage
|Part # 4014||20X WST-1 Solution, 1 ml||2-8°C|
|Part # 6010||Xanthine Oxidase Solution (XO), 20uL||2-8°C|
|Part # 3029||Assay Buffer, 20 mL||2-8°C|
|Part # 3030||Xanthine Oxidase Dilution Buffer, 10mL. (XO Dilution Buffer)||2-8°C|
|Part # 6011||SOD Enzyme, 30uL. See vial for acivity||2-8°C|
|Part # 9001||96 Well ELISA Plate, 1 plate||2-8°C|
|Part # 9002||Adhesive Plate Cover, Qty. 2||2-8°C|
|Malstrom, B., Andreasson, L., and Reinhammer, B. in The Enzymes. Byer, P., editor. XIIB, Academic Press, New York (1975).|
|Lontz, W., Sirsjo, J., Liu, W., Lindberg, M., Rollman, O., and G. (1976) Int. J. Cancer 17, 62–70. Torma, H. (1995) Free Radical Biol. Med. 18, 349–355.|
|Janero, D. R. (1995) CRC Crit. Revs. Food Sci. Nutr. 35, 65–81|
|Dominguez-Rodriguez, J.R. et al. (2001) Anticancer Res. 21:1869.|
|Zhao, Y. et al. (2001) Antioxid. Redox Signal 3:375.|
|# H. Ukeda, A. K. Sarker, D. Kawana and M. Sawamura, Anal. Sci., 15, 353 (1999).|
|Photocatalytic disinfection of marine bacteria using fluorescent light||http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6V73-4TGS7BN-1&_user=10&_coverDate=12%2F31%2F2008&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1287271047&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=a338bc47cd932e5bdf08ed85a516dd12||T.Y Leung, C. Y Chan,C. Hu, J.C Yu and p.k. wong||Water Research||vol 42 issue 19 – December 2008 pp 4827-4837|
|Vascular oxidative stress and nitric oxide depletion in HIV-1 transgenic rats are reversed by glutathione Restoration||http://ajpheart.physiology.org/content/294/6/H2792.short||Erik R. Kline, Dean J. Kleinhenz, Bill Liang, Sergey Dikalov, David M. Guidot, C. Michael Hart, Dean P. Jones, and Roy L. Sutliff||AJP – Heart||June 2008 vol. 294 no. 6, H2792-H2804|