- Cell permeability allow direct measurement of apoptosis and mitochondrial potential in live cells.
- Applications – Cells can be analyzed by Flow Cytometry, Fluorescent plate reader or Fluorescent microscopy.
- Incubate for 15 minutes, wash and measure.
- Add this reagent directly to live cells in your media of choice.
Detection of the mitochondrial permeability transition event provides an early indication of the initiation of cellular apoptosis. This process is typically defined as a collapse in the electrochemical gradient across the mitochondrial membrane, as measured by the change in the membrane potential (YD). Loss of mitochondrial (YD) is indicative of apoptosis and can be detected by a unique fluorescent cationic dye, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl- benzamidazolocarbocyanin iodide, commonly known as JC-1. This dye has been incorporated into the user-friendly kit for the simple and reproducible detection of the membrane potential (YD) event in apoptotic cells. The kit has been formatted for use on Flow cytometers, Fluorescent plate readers and Fluorescent Microscopes
Fig (A). Jurkat cells were cultured with DMSO for 2 hours. Cells were then stained with JC-1 Mitochondrial Membrane Potential Detection Kit for 15 minutes and analyzed by flow cytometry.
Fig (B). urkat cells were cultured with staurosporine for 2 hours. Cell were then stained with JC-1 Mitochondrial Membrane Potential Detection Kit for 15 minutes and analyzed by flow cytometry.