Key Benefits

  • Cell permeability allow direct measurement of apoptosis and mitochondrial potential in live cells.
  • Applications – Cells can be analyzed by Flow Cytometry, Fluorescent plate reader or Fluorescent microscopy.
  • Incubate for 15 minutes, wash and measure.
  • Add this reagent directly to live cells in your media of choice.

Additional information

Kit Size


Detection of the mitochondrial permeability transition event provides an early indication of the initiation of cellular apoptosis. This process is typically defined as a collapse in the electrochemical gradient across the mitochondrial membrane, as measured by the change in the membrane potential (YD). Loss of mitochondrial (YD) is indicative of apoptosis and can be detected by a unique fluorescent cationic dye, 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl- benzamidazolocarbocyanin iodide, commonly known as JC-1. This dye has been incorporated into the user-friendly kit for the simple and reproducible detection of the membrane potential (YD) event in apoptotic cells. The kit has been formatted for use on Flow cytometers, Fluorescent plate readers and Fluorescent Microscopes

Fig (A).
Jurkat cells were cultured with DMSO for 2 hours. Cells were then stained with JC-1 Mitochondrial Membrane Potential Detection Kit for 15 minutes and analyzed by flow cytometry.

Fig (B).
urkat cells were cultured with staurosporine for 2 hours. Cell were then stained with JC-1 Mitochondrial Membrane Potential Detection Kit for 15 minutes and analyzed by flow cytometry.

      http://www.jem.org/cgi/content/full/199/4/547Defects in Cell Growth Regulation by C 18:0-Ceramide and Longevity Assurance Gene 1 (LAG1) in Human Head and Neck Squamous Cell Carcinomas (HNSCC)Effects of a series of organosulfur compounds on mitotic arrest and induction of apoptosis in colon cancer cellsAdrenergic receptor-stimulated apoptosis in adult cardiac myocytes involves MMP-2-mediated disruption of β1 integrin signaling and mitochondrial pathwaySGLT-1-mediated glucose uptake protects intestinal epithelial cells against LPS-induced apoptosis and barrier defects: a novel cellular rescue mechanism?Ceramide induces mitochondrial abnormalities in insulin-secreting INS-1 cells: Potential mechanisms underlying ceramide-mediated metabolic dysfunction of the β cellOpposing effects of bovine papillomavirus type 1 E6 and E7 genes on Fas-mediated apoptosisResveratrol-caused apoptosis of human prostate carcinoma LNCaP cells is mediated via modulation of phosphatidylinositol 3'-kinase/Akt pathway and Bcl-2 family proteins
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    • Part# 4001: Lyophilized JC-1 Dye (Store at 2-8C)
    • Part# 3002: 10X Assay Buffer (Store at 2-8C)