Sarcosine is natural amino acid that is an important intermediate in the metabolism of choline. Sarcosine is an important component of proteins and plays a significant role in metabolic processes of living cells as a source of serine, creatine, purines and glutathione etc. Sarcosine is present in food sources like legumes, eggs, ham, turkey etc. It is used in a variety of industrial applications such as manufacturing of tooth paste and biodegradable surfactants. Sarcosine was recently reported to activate prostate cancer cells and as a possible marker for prostate cancer progression to metastasis(1). It is also used in adjunctive treatment for Schizophrenia (2) and depression (3).
Cell Technology’s Fluoro Sarcosine assay provides a reliable, sensitive fluorimetric method for the quantification of sarcosine in biological samples.
The Fluoro Sarcosine detection kit utilizes a non-fluorescent detection reagent, which is reduced via an enzyme-coupled reaction in the presence of sarcosine. A sarcosine standard curve is generated to quantify sarcosine in the samples.
- Easy to Use – No Wash Assay.
- Detection of Sarcosine in cells, serum, tissue extracts.
- Easy to Use 96-well Fluorescent Plate reader readout.
Kit contents and storage
|Part # 6024||Enzyme Mix, 1 Vial||-20°C|
|Part # 3056||Standard Curve Diluent, 40mL||2-8°C|
|Part # 4023||Sarcosine Detection Reagent, 1 Vial||2-8°C|
|Part # 7021||Sarcosine Standard, 1 Vial||2-8°C|
|Sreekumar, Arun; Poisson, Laila M.; Rajendiran, Thekkelnaycke M.; Khan, Amjad P.; Cao, Qi; Yu, Jindan; Laxman, Bharathi; Mehra, Rohit et al. (2009). “Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression”. Nature 457 (7231): 910. doi:10.1038/nature07762. PMID 19212411.|
|Lane H, Huang C, Wu P, Liu Y, Chang Y, Lin P, Chen P, Tsai G (2006). “Glycine transporter I inhibitor, N-methylglycine (sarcosine), added to clozapine for the treatment of schizophrenia”. Biol Psychiatry 60 (6): 645–9. doi:10.1016/j.biopsych.2006.04.005. PMID 16780811.|
|http://clinicaltrials.gov/ct2/show/NCT00977353 Clinicaltrials.gov “N-methylglycine (Sarcosine) Treatment for Depression”.|
Sarcosine + non-fluorescent detection reagent+ Enzyme Mix —–> fluorescent analog
Excitation: 530-570nm and Emission at 590-600
Table 1: We conducted spike and recovery experiments to estimate % recovery of sarcosine. Serum was spiked with sarcosine with the concentrations mentioned in the table above. The samples were processed as described in section IX of the protocol.
Table 2: We conducted spike and recovery experiments to estimate % recovery of sarcosine. Cell lysates were spiked with sarcosine with the concentrations mentioned in the table above. The samples were processed as described in section VIII: Mammalian Cell Preparation.
Table 3: Serum samples were diluted 1:5 in standard curve diluent. Jurkat and Daudi cells were prepared as described in the protocol. After the final wash cells were adjusted to 1X106 cells/mL in standard curve diluent. Sarcosine was measured as described in the protocol.
Figure 1. Sarcosine standard curve was generated as described in the protocol. R2= 0.998