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Fluoro NADP

Fluoro NADP Cell Technology Metabolic Assays
Fluoro NADP
Fluorescent NADP/NADPH Detection Kit - Detection of NADP/NADPH in tissue extracts /cell samples

$495.00$2,375.00

in stock
Product code: NADPH100
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Product Description

Introduction

Nicotinamide adenine dinucleotide phosphate (NADP+) is used in anabolic reactions, such as lipid and nucleic acid synthesis, which require NADPH as a reducing agent. NADPH is the reduced form of NADP+, and NADP+ is the oxidized form of NADPH. In cells, NADPH plays the role of a carrier of reducing power and is primarily involved in maintaining optimal redox metabolism. A simplified assay for the measurement of NAD and NADP is critical to understanding the roles of these pyridine nucleotides in normal and abnormal cells.

NADPH is produced in the oxidative phase of the pentose phosphate pathway in cells, a multifunctional pathway whose primary purpose is to generate reducing power in the form of NADPH. NADPH is a cofactor for enzymes that synthesize energy-rich molecules and provide the reducing equivalents for the oxidation-reduction involved in protecting the cell from the toxicity of reactive oxygen species (ROS) and NADPH oxidase-dependent ROS generation. Both NAD and NADP have been shown to influence hemoglobin affinity for oxygen in erythrocytes. In plant cells, NADPH is used as the reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis (1-2).

Cell Technology’s Fluoro NADP/NADPH provides a highly reliable, sensitive fluorometric assay for the quantification of NADP, NADPH and their ratio in biological samples.

Key Benefits

  • Detection of NADP/NADPH content in cells or tissue extracts.
  • Detection of NADP/NADPH levels in antioxidation and oxidative stress.
  • Detection of NADP/NADPH levels in cell death, energy metabolism, and mitochondria function.
  • Species Independent – NADP/NADPH detection in Bacterial, fungal and plant cells.
  • Highly Sensitive – Detects up to 4nM NADP and NADPH.
  • Highly Specific – No Cross reactivity with NAD/NADH.
  • Easy to Use – 96 well Fluorescent Plate reader readout.

Assay Principle

The Fluoro NADP/NADPH detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence NADPH to produce its fluorescent analog and NADP. NADP is further converted to NADPH via an enzyme-coupled reaction. The enzyme reaction specifically reacts with NADP/NADPH and not with NAD/NADH.

fluorescent_nadp_nadph_1

Figure 1. Comparison of NADPH & NADH Std Curves

fluorescent_nadp_nadph_2

Figure 2. Comparison of NADP & NADPH Std Curves

Kit contents and Long Term storage

ReagentPart#Temperature
Enzyme Mix, 170μLPart # 60222-8C
NADP/NADPH Lysis Solution, 12.5mLPart # 30502-8C
NADP Extraction Buffer, 22 mLPart # 3057 (Green label)2-8C
NADPH Extraction Buffer, 22 mLPart # 3058 (Magenta label)2-8C
Reaction Buffer, 11 mL.Part # 30492-8C
Standard curve diluent, 15 ml.Part # 30522-8C
NADPH Detection reagent, 110 μLPart # 4019-20C
3X Substrate Mix, 6mLPart # 3048-20C
NADPH Standard, 3 vials driedPart # 7014-20C
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