Fluoro NAD

$550.00$2,645.00

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Description

Introduction

The role of pyridine nucleotides (NAD/NADH) metabolism in health, is of continual and increased interest. A growing amount of evidence supports the fact that NAD metabolism regulates important biological effect including life span. NAD, through poly-ADP-ribosyl polymerase (PARP), mono-ADP-ribosyltransferase (ARTs) and recently characterized sirtuin enzymes, exerts potential biological effects. These enzymes modify proteins to regulate their function via ADP-ribosylation or deacetylation and are involved in several pathways including apoptosis, DNA repair, senescence and endocrine signaling. This suggests that either the enzymes or NAD could be an important therapeutical target 1.

Key Benefits

  • Detection of NAD/NADH content in cells or tissue extracts.
  • Detection of NAD/NADH levels in apoptosis, metabolism, proliferation, DNA repair, senescence, endocrine signaling and life span.
  • NAD/NADH detection in Bacterial, fungal and plant cells.
  • Fluorescent 96 well Plate Reader Readout (excitation: 530-570nm and emission at 590-600nm).

Additional information

Kit Size

100, 500

The Fluoro NAD/NADH detection kit utilizes a non-fluorescent detection reagent, which is reduced in the presence NADH to produce its fluorescent analog and NAD. NAD is further converted to NADH via an enzyme coupled reaction. The enzyme reaction specifically reacts with NAD/NADH and not with NADP/NADPH
Reaction:

1. NADH + non-fluorescent detection reagent+ electron    coupler fluorescent

analog + NAD

2. NAD + enzyme coupled reaction   NADH (then proceeds to reaction 1).

excitation: 530-570nm and emission at 590-600nm

fluorescent_nad_nadh

Figure 1. NADH standard curve titrated in NAD/NADH lysis buffer. Incubation time = 1 hour.

fluorescent_nadh_assay

Figure 2. NAD/NADH Assay showing no cross-reactivity with NADPH. Incubation time = 1 hour.

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    • Anthony A. Sauve NAD+ and Vitamin B3: From metabolism to therapies J. Pharmacol. Exp. Ther. 2007 : jpet.107.120758v1
    • Part# 6021: Enzyme Mix ('-20C)
    • Part# 4018: NADH Detection Reagent ('-20C)
    • Part# 7013: NADH Standard ('-20C)
    • Part# 3044: NAD/NADH Reaction Buffer (2-8 C)
    • Part# 3045: NAD/NADH Lysis Buffer (2-8 C)
    • Part# 3046: NAD Extraction Buffer (2-8 C)
    • Part# 3047: NADH Extraction Buffer (2-8 C)
    • Part# 3051: NADH Standard Diluent (2-8 C)