- Can monitor multiple time points to follow kinetics.
- One-step, no wash assay.
- Adaptable for High Throughput format.
- Highly Sensitive.
- Applications – Fluorescent Plate Reader or Absorbance Plate Reader.
- Detection of MPO activity in neutrophils and macrophages.
- Detection of PMN infiltration in tissue samples (inflammation and innate host defense mechanisms).
- Acute and chronic inflammatory disorders due to oxidative tissue damage.
- MPO activity in acute and chronic manifestations of cardiovascular disease.
Figure 1. MPO standard curve was serially diluted in 1X Reaction Buffer. Reaction cocktail (RC) was prepared as described (without EPO inhibitor). Next 50uL of MPO standard and 50uL of RC was added to individual well of a 96 well black plates. The plate was incubated at room and temperature in the dark. Data collected Ex:530nm Em:590nm
|Use of a hanging-weight system for liver ischemic preconditioning in mice||http://ajpgi.physiology.org/cgi/content/abstract/294/6/G1431||Hart, Much, Kohler et.al||Am J Physiol Gastrointest Liver Physiol||294: G1431-G1440, 2008|
|Extracellular Adenosine Production by Ecto-5′-Nucleotidase Protects During Murine Hepatic Ischemic Preconditioning||http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WFX-4T4HP30-3&_user=10&_coverDate=11%2F30%2F2008&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1245872620&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=0348e5d389c76f43fa0ca609f91a05f7||M. Hart, C. Much, I. Gorzolla, J. Schittenhelm, D. Kloor, G. Stahl, H. Eltzschig||Gastroenterology||Volume 135, Issue 5, Pages 1739-1750.e3|
|Inflammatory Biomarkers of Sulfur Mustard Analog 2-Chloroethyl Ethyl Sulfide–Induced Skin Injury in SKH-1 Hairless Mice||http://toxsci.oxfordjournals.org/cgi/content/abstract/108/1/194||Neera Tewari-Singh, Sumeet Rana, Mallikarjuna Gu, Arttatrana Pal, David J. Orlicky, Carl W. White and Rajesh Agarwal||Toxicological Sciences||2009 108(1):194-206|
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|Andrews, P.C., Parnes, C. & Krinsky, N.I. (1984) Comparison of myeloperoxidase and hemi-myeloperoxidase with respect to catalysis, regulation, and bacterial activity. Arch. Biochem. Biophys. 228, 439–442.|
|Mingjie Zhou, Zhenjun Diwu, Nataliya Panchuk-Voloshina and Richard P. Haugland. A Stable Nonfluorescent Derivative of Resorufin for the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte NADPH Oxidase and Other Oxidases. Anal Biochem 253, 162 (1997).|
|J. G. Mohanty, Jonathan S. Jaffe, Edward S. Schulman and Donald G. Raible. A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative. J. Immunol Methods 202, 133 (1997).|
|Tatyana V. Votyakova and Ian J. Reynolds. Membrane Potential dependent and -independent production of reactive oxygen species by rat brain mitochondria. J Neurochem 79, 266 (2001).|
|Chun Song, Abu B. Al-Mehdi, and Aron B. Fisher. An immediate endothelial cell signaling response to lung ischemia. Am J Physiol Lung Cell Mol Physiol 281, L993 (2001).|
|Samantha C. Richer and W.C.L. Ford. A critical investigation of NADPH oxidase activity in human spermatozoa. Mol Hum Reprod 7, 237 (2001).|
|William G. Gutheil, Miglena E. Stefanova and Robert A. Nicholas. Fluorescent Coupled Enzyme Assays for -Alanine: Application to Penicillin-Binding Protein and Vancomycin Activity Assays. Anal Biochem 287, 196 (2000).|
|Dominik Peus, Remus A. Vasa, Astrid Beyerle, Alexander Meves, Carsten Krautmacher and Mark R. Pittelkow. UVB Activates ERK1/2 and p38 Signaling Pathways via Reactive Oxygen Species in Cultured Keratinocytes. J Invest Dermatol 112, 751 (1999).|
|Tatyana V.Votyakova ,Ian J.Reynolds. Detection of hydrogen peroxide with Amplex Red:interference by NADH and reduced glutathione auto-oxidation. Archives of Biochemistry and Biophysics, 431: 138-144 (2004)..|
|Elizabeth Forbes, Tosei Murase, Ming Yang, Klaus I. Matthaei,*James J. Lee, Nancy A. Lee, Paul S. Foster, and Simon P. Hogan. Immunopathogenesis of Experimental Ulcerative Colitis Is Mediated by Eosinophil Peroxidase. The Journal of Immunology, 2004, 172:5664–5675.|
|Part# 4007||Detection Reagent, 1 Vial||-20C|
|Part# 3002||10X Assay Buffer, 60mL||2-8C|
|Part# 3012||Hydrogen Peroxide, 1000µL of a Stabilized 3% Solution||2-8C|
|Part# 6015||Myeloperoxidase, 1 Vial at 30Units/mL||2-8C|