Fluoro MPO




Key Benefits

  • Can monitor multiple time points to follow kinetics.
  • One-step, no wash assay.
  • Adaptable for High Throughput format.
  • Highly Sensitive.
  • Applications – Fluorescent Plate Reader or Absorbance Plate Reader.

Additional information

Kit Size



  • Detection of MPO activity in neutrophils and macrophages.
  • Detection of PMN infiltration in tissue samples (inflammation and innate host defense mechanisms).
  • Acute and chronic inflammatory disorders due to oxidative tissue damage.
  • MPO activity in acute and chronic manifestations of cardiovascular disease.


Figure 1. MPO standard curve was serially diluted in 1X Reaction Buffer. Reaction cocktail (RC) was prepared as described (without EPO inhibitor). Next 50uL of MPO standard and 50uL of RC was added to individual well of a 96 well black plates. The plate was incubated at room and temperature in the dark. Data collected Ex:530nm Em:590nm

Readable Documents

Title Name
Protocol Protocol.pdf
Datasheet Datasheet.pdf
msds msds.pdf

Kit contents and storage

Part# Reagent Temperature
Part# 4007 Detection Reagent, 1 Vial -20°C
Part# 3002 10X Assay Buffer, 60mL 2-8°C
Part# 3012 Hydrogen Peroxide, 1000µL of a Stabilized 3% Solution 2-8°C
Part# 6015 Myeloperoxidase, 1 Vial at 30Units/mL 2-8°C



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Mark B. Hampton, Anthony J. Kettle, and Christine C. Winterbourn. Inside the Neutrophil Phagosome: Oxidants, Myeloperoxidase, and Bacterial Killing. Blood, Vol. 92 No. 9 (November 1), 1998: pp. 3007-3017.
Andrews, P.C., Parnes, C. & Krinsky, N.I. (1984) Comparison of myeloperoxidase and hemi-myeloperoxidase with respect to catalysis, regulation, and bacterial activity. Arch. Biochem. Biophys. 228, 439–442.
Mingjie Zhou, Zhenjun Diwu, Nataliya Panchuk-Voloshina and Richard P. Haugland. A Stable Nonfluorescent Derivative of Resorufin for the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte NADPH Oxidase and Other Oxidases. Anal Biochem 253, 162 (1997).
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Tatyana V.Votyakova ,Ian J.Reynolds. Detection of hydrogen peroxide with Amplex Red:interference by NADH and reduced glutathione auto-oxidation. Archives of Biochemistry and Biophysics, 431: 138-144 (2004)..
Elizabeth Forbes, Tosei Murase, Ming Yang, Klaus I. Matthaei,*James J. Lee, Nancy A. Lee, Paul S. Foster, and Simon P. Hogan. Immunopathogenesis of Experimental Ulcerative Colitis Is Mediated by Eosinophil Peroxidase. The Journal of Immunology, 2004, 172:5664–5675.



Title File Link Author(s) Journal Year; Edition:Pages
Use of a hanging-weight system for liver ischemic preconditioning in mice http://ajpgi.physiology.org/cgi/content/abstract/294/6/G1431 Hart, Much, Kohler et.al Am J Physiol Gastrointest Liver Physiol 294: G1431-G1440, 2008
Extracellular Adenosine Production by Ecto-5′-Nucleotidase Protects During Murine Hepatic Ischemic Preconditioning http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WFX-4T4HP30-3&_user=10&_coverDate=11%2F30%2F2008&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1245872620&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=0348e5d389c76f43fa0ca609f91a05f7 M. Hart, C. Much, I. Gorzolla, J. Schittenhelm, D. Kloor, G. Stahl, H. Eltzschig Gastroenterology Volume 135, Issue 5, Pages 1739-1750.e3
Inflammatory Biomarkers of Sulfur Mustard Analog 2-Chloroethyl Ethyl Sulfide–Induced Skin Injury in SKH-1 Hairless Mice http://toxsci.oxfordjournals.org/cgi/content/abstract/108/1/194 Neera Tewari-Singh, Sumeet Rana, Mallikarjuna Gu, Arttatrana Pal, David J. Orlicky, Carl W. White and Rajesh Agarwal Toxicological Sciences 2009 108(1):194-206


Myeloperoxidase (MPO) is a highly cationic glycosolated hemoprotein that has a molecular weight of 144kD. The hemoprotein consists of two dimers linked via a disulfide bridge. Each dimmer is composed of a heavy (53kD) and light (15kD) subunit. Each heavy chain contains an independently acting protoporphyrin group containing a central iron (1-5). MPO is present in the azurophilic granules of polymorphonuclear leukocytes (PMNs) and is unique to neutrophils and monocytes. However, monocytes contain only one third of the MPO found in PMN’s. MPO utilizes H202 produced by the neutrophils to oxidize a varity of aromatic compounds to give substrate radicals for bactericidal activity (4 review). This enzyme is unique however in that it can oxidize chloride ions to produce a strong nonradical oxidant,HOCl. HOCl is the most powerfull bactericidal produced by neutrophils (4 review). Excessive production of these radicals can cause oxidative stress leading to oxidative tissue injury.

H2O2 + Detection reagent (non-fluorescent)+ MPO ———> fluorescent analog

Excitation:530-571nm; Emission: 590-600nm