Fluoro Hydroxyl/ Peroxynitrite





A new novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1.

Key Benefits

  • Quenched Cell permeable dye.
  • One Step, No wash Homogenous assay.
  • Adaptable to High throughput assay platforms.
  • Can monitor multiple time points to follow real time kinetics.
  • Non-destructive cell based assay allows monitoring of additional parameters.
  • Applications – Fluorescent plate reader / Flow Cytometery / Fluorescent Microscopy.

Additional information

Kit Size



      Paradoxical effect of l-arginine: Acceleration of endothelial cell senescenceThe neuroprotective effect of ladostigil against hydrogen peroxide-mediated cytotoxicityGeneration of highly-reactive oxygen species is closely related to hair cell damage in rat organ of corti treated with gentamicinThe Novel Cholinesterase–Monoamine Oxidase Inhibitor and Antioxidant, Ladostigil, Confers Neuroprotection in Neuroblastoma Cells and Aged RatsPost-translational activation of human phenylalanine 4-monooxygenase from an endobiotic to a xenobiotic enzyme by reactive oxygen and reactive nitrogen species
    • Ken-ichi Setsukinai, Yasuteru Urano, Katsuko Kakinuma, Hideyuki J. Majima , and Tetsuo Nagano. Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 278, No. 5, Issue of January 31, pp. 3170–3175, 2003
    • Part# 4012: HPF, 1 Vial (2-8C)

    Not for Sale in Japan

    *Test size will depend on dilution and volume used per test. For example:

    Dilution of HPF Volume of test Numer of tests
    1:500 (10uM final) 0.2 mL 147
    1: 1000 (5uM final) 0.2 mL 294