Fluoro Hydroxyl/ Peroxynitrite




A new novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1.

Key Benefits

  • Quenched Cell permeable dye.
  • One Step, No wash Homogenous assay.
  • Adaptable to High throughput assay platforms.
  • Can monitor multiple time points to follow real time kinetics.
  • Non-destructive cell based assay allows monitoring of additional parameters.
  • Applications – Fluorescent plate reader / Flow Cytometery / Fluorescent Microscopy.

Additional information

Kit Size



TitleFileLinkAuthor(s)JournalYear; Edition:Pages
Paradoxical effect of l-arginine: Acceleration of endothelial cell senescencehttp://www.sciencedirect.com/science/article/pii/S0006291X09012376Ortunato Scalera, Ellen I. Closs, Eva Flick, Jens Martens-Lobenhoffer, Jean P. Boissel, Uwe Lendeckel, Anke Heimburg and Stefanie M. Bode-BögerBiochemical and Biophysical Research CommunicationsVolume 386, Issue 4, 4 September 2009, Pages 650-655
The neuroprotective effect of ladostigil against hydrogen peroxide-mediated cytotoxicityhttp://www.sciencedirect.com/science/article/pii/S0009279708003086Orly Weinreb, Orit Bar-Am, Tamar Amit, Noam Drigues, Yotam Sagi and Moussa B.H. YoudimChemico-Biological InteractionsVolume 175, Issues 1-3, 25 September 2008, Pages 318-326
Generation of highly-reactive oxygen species is closely related to hair cell damage in rat organ of corti treated with gentamicinhttp://www.sciencedirect.com/science/article/pii/S0306452209003352Y.H. Choung, A. Taura, K. Pak, S.J. Choi, M. Masuda and A.F. RyanNeuroscienceVolume 161, Issue 1, 16 June 2009, Pages 214-226
The Novel Cholinesterase–Monoamine Oxidase Inhibitor and Antioxidant, Ladostigil, Confers Neuroprotection in Neuroblastoma Cells and Aged Ratshttp://www.springerlink.com/content/tgg06484h1142777/Orit Bar-Am, Orly Weinreb, Tamar Amit and Moussa B. H. YoudimJournal of Molecular NeuroscienceVolume 37, Number 2, 135-145, DOI: 10.1007/s12031-008-9139-6
Post-translational activation of human phenylalanine 4-monooxygenase from an endobiotic to a xenobiotic enzyme by reactive oxygen and reactive nitrogen specieshttp://informahealthcare.com/doi/abs/10.3109/00498251003675207A. Antypa, C. Rebello, A. Biernacka, K. Krajewski, J. Cassam, S. C. Mitchell, G. B. Steventondoi:10.3109/00498251003675207May 2010, Vol. 40, No. 5 , Pages 319-330
Ken-ichi Setsukinai, Yasuteru Urano, Katsuko Kakinuma, Hideyuki J. Majima , and Tetsuo Nagano. Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 278, No. 5, Issue of January 31, pp. 3170–3175, 2003
Part# 4012HPF, 1 Vial2-8C

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