Fluoro Hydroxyl/ Peroxynitrite





A new novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1.

Key Benefits

  • Quenched Cell permeable dye.
  • One Step, No wash Homogenous assay.
  • Adaptable to High throughput assay platforms.
  • Can monitor multiple time points to follow real time kinetics.
  • Non-destructive cell based assay allows monitoring of additional parameters.
  • Applications – Fluorescent plate reader / Flow Cytometery / Fluorescent Microscopy.

Additional information

Kit Size




Readable Documents

Title Name
Protocol Fluoro HPF Protocol.pdf
Datasheet Fluoro HPF Datasheet.pdf
msds msds Fluoro HPF.pdf

Kit contents and storage

Part# Reagent Temperature
Part# 4012 HPF, 1 Vial 2-8°C



Ken-ichi Setsukinai, Yasuteru Urano, Katsuko Kakinuma, Hideyuki J. Majima , and Tetsuo Nagano. Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 278, No. 5, Issue of January 31, pp. 3170–3175, 2003



Title File Link Author(s) Journal Year; Edition:Pages
Paradoxical effect of l-arginine: Acceleration of endothelial cell senescence http://www.sciencedirect.com/science/article/pii/S0006291X09012376 Ortunato Scalera, Ellen I. Closs, Eva Flick, Jens Martens-Lobenhoffer, Jean P. Boissel, Uwe Lendeckel, Anke Heimburg and Stefanie M. Bode-Böger Biochemical and Biophysical Research Communications Volume 386, Issue 4, 4 September 2009, Pages 650-655
The neuroprotective effect of ladostigil against hydrogen peroxide-mediated cytotoxicity http://www.sciencedirect.com/science/article/pii/S0009279708003086 Orly Weinreb, Orit Bar-Am, Tamar Amit, Noam Drigues, Yotam Sagi and Moussa B.H. Youdim Chemico-Biological Interactions Volume 175, Issues 1-3, 25 September 2008, Pages 318-326
Generation of highly-reactive oxygen species is closely related to hair cell damage in rat organ of corti treated with gentamicin http://www.sciencedirect.com/science/article/pii/S0306452209003352 Y.H. Choung, A. Taura, K. Pak, S.J. Choi, M. Masuda and A.F. Ryan Neuroscience Volume 161, Issue 1, 16 June 2009, Pages 214-226
The Novel Cholinesterase–Monoamine Oxidase Inhibitor and Antioxidant, Ladostigil, Confers Neuroprotection in Neuroblastoma Cells and Aged Rats http://www.springerlink.com/content/tgg06484h1142777/ Orit Bar-Am, Orly Weinreb, Tamar Amit and Moussa B. H. Youdim Journal of Molecular Neuroscience Volume 37, Number 2, 135-145, DOI: 10.1007/s12031-008-9139-6
Post-translational activation of human phenylalanine 4-monooxygenase from an endobiotic to a xenobiotic enzyme by reactive oxygen and reactive nitrogen species http://informahealthcare.com/doi/abs/10.3109/00498251003675207 A. Antypa, C. Rebello, A. Biernacka, K. Krajewski, J. Cassam, S. C. Mitchell, G. B. Steventon doi:10.3109/00498251003675207 May 2010, Vol. 40, No. 5 , Pages 319-330