Fluoro H202



Key Benefits

  • Quick 10 minute assay.
  • Can monitor multiple time points to follow kinetics.
  • Dual mode, can detect H202 or peroxidase activity.
  • One-step, no wash assay.
  • Adaptable for High Throughput format.
  • Non-destructive cell based assay allows monitoring of additional parameters.
  • Applications – Fluorescent Plate Reader.

Additional information

Kit Size


The Fluoro H2O2 detection kit utilizes a non-fluorescent detection reagent, to detect H2O2. H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.

The detection reagent can be utilized to detect H2O2 release from cells or enzyme coupled reactions (1-7)


Figure 1. Hydrogen Peroxide titrated in 1X reaction Buffer and measured using the Fluoro H2O2 Kit.

TitleFileLinkAuthor(s)JournalYear; Edition:Pages
Deficiency of the Bax gene attenuates denervation-induced apoptosishttp://www.springerlink.com/content/p1n841w124828181/P. M. Siu and S. E. AlwayApoptosis JournalVol 11/No 6 June 2006
Manganese chloride stimulates rat microglia to release hydrogen peroxidehttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCR-4P2S92J-1&_user=10&_coverDate=09%2F10%2F2007&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_searchStrId=1722767089&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=7f28a0171643f2629b142edec039c748&searchtype=aPing Zhang, Angela Hatter and Bin LiuToxicology LettersVolume 173, Issue 2, 10 September 2007, Pages 88-100 -doi:10.1016/j.toxlet.2007.06.013
Superoxide dismutase protects against apoptosis and alveolar enlargement induced by ceramidehttp://ajplung.physiology.org/content/295/1/L44.shortIrina Petrache, Terry R. Medler, Amy T. Richter, Krzysztof Kamocki, Ugonma Chukwueke, Lijie Zhen, Yuan Gu, Jeremy Adamowicz, Kelly S. Schweitzer,1 Walter C. Hubbard, Evgeny V. Berdyshev, Giuseppe Lungarella, and Rubin M. TuderAmerican Journal of Physiology, Lung PhysiolJuly 2008, Vol 295, No 1 L44-L53
Dual Role of Vitamin C Utilization in NO2-Induced Oxidative Stress in Lung Tissues of Micehttp://www.springerlink.com/content/j0870103v5820t78/Yingyi Zhang, Chunyan Ma, Yuhuan Xiao, Chang Liu, Qiang Xu, Yan Wang, Xin Xu and Lin HaoBulletin of Environmental Contamination and ToxicologyVol 84, Number 6, 662-666 - DOI: 10.1007/s00128-010-0021-1
Cigarette Smoke-Generated Reactive Oxygen Species Impair Adenosine-Mediated Wound Closure in Bronchial Epithelial Cellshttp://ajrccm.atsjournals.org/cgi/content/citation/179/1_MeetingAbstracts/A2382DS Allen-Gipson, JC Jarrell, and H Alvarez-RamirezAmerican Journal of Respiratory and Critical care medicine2009; 179: A2382
Benzyl isothiocyanate-mediated generation of reactive oxygen species causes cell cycle arrest and induces apoptosis via activation of MAPK in human pancreatic cancer cellshttp://carcin.oxfordjournals.org/cgi/content/abstract/30/10/1744Ravi P. Sahu, Ruifen Zhang, Sanjay Batra, Yan Shi and Sanjay K. SrivastavaCarcinogenesis2009 30(10):1744-1753
Vibration Disrupts Vascular Function in a Model of Metabolic Syndromehttp://www.jstage.jst.go.jp/article/indhealth/47/5/47_533/_articleKristine Krajnak, Stacey Waugh, Claud Johnson, Roger Miller, Meagan KiedrowskiIndustrial HealthVol 47 (2009), No 5, pp 533-542
Inhibition of xanthine oxidase reduces oxidative stress and improves skeletal muscle function in response to electrically stimulated isometric contractions in aged micehttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T38-52JVX0M-1&_user=10&_coverDate=04%2F07%2F2011&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_searchStrId=1722912834&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=daa9b274fb07dfe348917b389f76c96b&searchtype=aMichael J. Ryan, Janna R. Jackson, Yanlei Hao, Stephen S. Leonard and Stephen E. AlwayFree Radical Biology and MedicineApril 2011, doi:10.1016/j.freeradbiomed.2011.04.002
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J Neurochem 79, 266 (2001)
Am J Physiol Lung Cell Mol Physiol 281, L993 (2001)
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J Invest Dermatol 112, 751 (1999)
Part # 30115X Reaction Buffer: 20 ml buffer, pH 7.42-8°C
Part # 4007Detection reagent: One vial for 500 assays.2-8°C
Part # 3012Hydrogen Peroxide: 200µL of a stabilized 3% solution.2-8°C
Part # 6004Horseradish Peroxidase: 18.9 Units of enzyme2-8°C

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