- Additional information
- Assay Principle
- Readable Documents
- Kit Contents Long Term Storage
- Quick 10 minute assay.
- Can monitor multiple time points to follow kinetics.
- Dual mode, can detect H202 or peroxidase activity.
- One-step, no wash assay.
- Adaptable for High Throughput format.
- Non-destructive cell based assay allows monitoring of additional parameters.
- Applications – Fluorescent Plate Reader.
The Fluoro H2O2 detection kit utilizes a non-fluorescent detection reagent, to detect H2O2. H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.
The detection reagent can be utilized to detect H2O2 release from cells or enzyme coupled reactions (1-7)
Figure 1. Hydrogen Peroxide titrated in 1X reaction Buffer and measured using the Fluoro H2O2 Kit.
- Deficiency of the Bax gene attenuates denervation-induced apoptosisManganese chloride stimulates rat microglia to release hydrogen peroxideSuperoxide dismutase protects against apoptosis and alveolar enlargement induced by ceramideDual Role of Vitamin C Utilization in NO2-Induced Oxidative Stress in Lung Tissues of MiceCigarette Smoke-Generated Reactive Oxygen Species Impair Adenosine-Mediated Wound Closure in Bronchial Epithelial CellsBenzyl isothiocyanate-mediated generation of reactive oxygen species causes cell cycle arrest and induces apoptosis via activation of MAPK in human pancreatic cancer cellsVibration Disrupts Vascular Function in a Model of Metabolic SyndromeInhibition of xanthine oxidase reduces oxidative stress and improves skeletal muscle function in response to electrically stimulated isometric contractions in aged mice
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- Part # 3011: 5X Reaction Buffer: 20 ml buffer, pH 7.4 (2-8°C)
- Part # 4007: Detection reagent: One vial for 500 assays. (2-8°C)
- Part # 3012: Hydrogen Peroxide: 200µL of a stabilized 3% solution. (2-8°C)
- Part # 6004: Horseradish Peroxidase: 18.9 Units of enzyme (2-8°C)