Fluoro H202

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Description

Key Benefits

  • Quick 10 minute assay.
  • Can monitor multiple time points to follow kinetics.
  • Dual mode, can detect H202 or peroxidase activity.
  • One-step, no wash assay.
  • Adaptable for High Throughput format.
  • Non-destructive cell based assay allows monitoring of additional parameters.
  • Applications – Fluorescent Plate Reader.

Additional information

Kit Size

500

ASSAY PRINCIPLE

The Fluoro H2O2 detection kit utilizes a non-fluorescent detection reagent, to detect H2O2. H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.

The detection reagent can be utilized to detect H2O2 release from cells or enzyme coupled reactions (1-7)

flouro4-f1

Figure 1. Hydrogen Peroxide titrated in 1X reaction Buffer and measured using the Fluoro H2O2 Kit.

Readable Documents

Title Name
Protocol Fluoro H2O2 Protocol.pdf
Datasheet Fluoro H2O2 Datasheet.pdf
msds msds Fluoro H2O2.pdf

Kit contents and storage

Part# Reagent Temperature
Part # 3011 5X Reaction Buffer: 20 ml buffer, pH 7.4 2-8°C
Part # 4007 Detection reagent: One vial for 500 assays. 2-8°C
Part # 3012 Hydrogen Peroxide: 200µL of a stabilized 3% solution. 2-8°C
Part # 6004 Horseradish Peroxidase: 18.9 Units of enzyme 2-8°C

 

References

Anal Biochem 253, 162 (1997)
J. Immunol Methods 202, 133 (1997)
J Neurochem 79, 266 (2001)
Am J Physiol Lung Cell Mol Physiol 281, L993 (2001)
Mol Hum Reprod 7, 237 (2001)
Willingham MC (1999) Cytochemical methods for the detection of apoptosis. J Histochem Cytochem 47:1101–1109
J Invest Dermatol 112, 751 (1999)

 

Citations

Title File Link Author(s) Journal Year; Edition:Pages
Deficiency of the Bax gene attenuates denervation-induced apoptosis http://www.springerlink.com/content/p1n841w124828181/ P. M. Siu and S. E. Alway Apoptosis Journal Vol 11/No 6 June 2006
Manganese chloride stimulates rat microglia to release hydrogen peroxide http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCR-4P2S92J-1&_user=10&_coverDate=09%2F10%2F2007&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_searchStrId=1722767089&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=7f28a0171643f2629b142edec039c748&searchtype=a Ping Zhang, Angela Hatter and Bin Liu Toxicology Letters Volume 173, Issue 2, 10 September 2007, Pages 88-100 -doi:10.1016/j.toxlet.2007.06.013
Superoxide dismutase protects against apoptosis and alveolar enlargement induced by ceramide http://ajplung.physiology.org/content/295/1/L44.short Irina Petrache, Terry R. Medler, Amy T. Richter, Krzysztof Kamocki, Ugonma Chukwueke, Lijie Zhen, Yuan Gu, Jeremy Adamowicz, Kelly S. Schweitzer,1 Walter C. Hubbard, Evgeny V. Berdyshev, Giuseppe Lungarella, and Rubin M. Tuder American Journal of Physiology, Lung Physiol July 2008, Vol 295, No 1 L44-L53
Dual Role of Vitamin C Utilization in NO2-Induced Oxidative Stress in Lung Tissues of Mice http://www.springerlink.com/content/j0870103v5820t78/ Yingyi Zhang, Chunyan Ma, Yuhuan Xiao, Chang Liu, Qiang Xu, Yan Wang, Xin Xu and Lin Hao Bulletin of Environmental Contamination and Toxicology Vol 84, Number 6, 662-666 – DOI: 10.1007/s00128-010-0021-1
Cigarette Smoke-Generated Reactive Oxygen Species Impair Adenosine-Mediated Wound Closure in Bronchial Epithelial Cells http://ajrccm.atsjournals.org/cgi/content/citation/179/1_MeetingAbstracts/A2382 DS Allen-Gipson, JC Jarrell, and H Alvarez-Ramirez American Journal of Respiratory and Critical care medicine 2009; 179: A2382
Benzyl isothiocyanate-mediated generation of reactive oxygen species causes cell cycle arrest and induces apoptosis via activation of MAPK in human pancreatic cancer cells http://carcin.oxfordjournals.org/cgi/content/abstract/30/10/1744 Ravi P. Sahu, Ruifen Zhang, Sanjay Batra, Yan Shi and Sanjay K. Srivastava Carcinogenesis 2009 30(10):1744-1753
Vibration Disrupts Vascular Function in a Model of Metabolic Syndrome http://www.jstage.jst.go.jp/article/indhealth/47/5/47_533/_article Kristine Krajnak, Stacey Waugh, Claud Johnson, Roger Miller, Meagan Kiedrowski Industrial Health Vol 47 (2009), No 5, pp 533-542
Inhibition of xanthine oxidase reduces oxidative stress and improves skeletal muscle function in response to electrically stimulated isometric contractions in aged mice http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T38-52JVX0M-1&_user=10&_coverDate=04%2F07%2F2011&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_searchStrId=1722912834&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=daa9b274fb07dfe348917b389f76c96b&searchtype=a Michael J. Ryan, Janna R. Jackson, Yanlei Hao, Stephen S. Leonard and Stephen E. Alway Free Radical Biology and Medicine April 2011,