Fluoro ATP
$345.00 – $1,595.00
- Description
- Additional information
- Introduction
- Reaction
- Readable Documents
- Kit contents and storage
- References
- Key Benefits
Description
Introduction
Adenosine Triphosphate (ATP) is an organic molecule which is exclusively formed in the mitochondria. Vritually all living systems store energy in the phosphate bonds of ATP and plays a central role in cellular metabolism and energy transfer reactions. ATP and be de-phosphorylation to ADP and further de-phosphorylation to AMP. This cycling of ATP is central to transferring potential (thermodynamic) energy from one source to another. There are several genetic disorders that effect the generation of ATP in the mitochondria (1-3).
Cell Technology’s Fluoro ATP assay provides a reliable, sensitive fluorimetric assay for the quantification of ATP in biological samples.
Key Benefits
- Detection of ATP in cells or tissue extracts.
- Detection of ATP in cell death, energy metabolism, mitochondria function.
- ATP measurement in ATP consuming enzymes such as Kinases and ATPases.
- ATP detection in Bacterial, Fungal and Plant Cells.
- Fluorescent 96 well Plate Reader Readout (excitation: 530-570nm and emission at 590-600nm).
Additional information
| Kit Size | 100, 500 |
|---|
Introduction
Adenosine Triphosphate (ATP) is an organic molecule that is exclusively formed in the mitochondria. Virtually all living systems store energy in the phosphate bonds of ATP and plays a central role in cellular metabolism and energy transfer reactions. ATP and be de-phosphorylation to ADP and further de-phosphorylation to AMP. This cycling of ATP is central to transferring potential (thermodynamic) energy from one source to another. There are several genetic disorders that affect the generation of ATP in the mitochondria (1-3).
Cell Technology’s Fluoro ATP assay provides a reliable, sensitive fluorimetric assay for the quantification of ATP in biological samples.
Reaction
1. ATP + Enzyme Coupled Reaction + Non-Fluorescent Detection Reagent → Fluorescent Analog + AMP
Detection: Excitation: 530-570nm and Emission at 590-600nm
2. 50µL of sample or ATP Standard
+
50µL of Enzyme Reaction Cocktail
↓
Incubate 30 – 60 minutes; RT; DARK
Read on Plate Reader: Excitation: 530-570nm
Emission at 590-600nm
Figure 1. ATP vs ADP Standard Curve fitted with linear regression.
|
ATP Spike |
% Recovery |
% Recovery |
|
no NEM |
40mM NEM | |
|
25.5 |
170 |
95.85 |
|
8.35 |
230 |
102 |
| 2.154 | 227 |
86.75 |
Table 1. ATP was spiked into Jurkat cell samples in Substrate Buffer (Part# 3046) with or without 40mM NEM (Part# 7026). % Recovery was determined via linear regression from ATP standard curve. N=3 per sample.
Readable Documents
| Title | Name |
|---|---|
| msds | msds.fluoroATP.pdf |
| Protocol | Fluoro-ATP-Protocol.pdf |
| Datasheet | Fluoro-ATP-Datasheet.pdf |
Kit contents and storage
| 6032 | Enzyme Mix 25X | ‘-20°C |
| 3065 | Substrate Buffer | ‘-20°C |
| 4028 | Detection Reagent 100X | ‘-20°C |
| 7027 | ADP Standard 2.5 mM | ‘-20°C |
| 7026 | NEM | ‘-20°C |
References
| Mitchell, P., Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism. Nature, 191, 144–148 (1961). |
| Alirol, E., and Martinou, J.C., Mitochondria and cancer: is there a morphological connection? Oncogene, 25, 4706–4716 (2006). |
| Carrozzo, R. et al., Infantile mitochondrial disorders. Biosci. Rep., 27, 105–112 (2007). |
Key Benefits
- Detection of ATP in cells or tissue extracts.
- Detection of ATP in cell death, energy metabolism, mitochondria function.
- ATP measurement in ATP consuming enzymes such as Kinases and ATPases.
- ATP detection in Bacterial, Fungal and Plant Cells.
- Fluorescent 96 well Plate Reader Readout (excitation: 530-570nm and emission at 590-600nm).
