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aCella Cyclic GMP Cell Technology Ultra Sensitive Luminescence Kits
Cyclic GMP
aCella™ - cGMP: Cyclic GMP Chemiluminescent Immunoassay Kit

$375.00

in stock
Product code: CLACGMP100
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Product Description

Key Benefits

  • Ultra-sensitive – Measures as little as < 1 fmol cGMP per sample.
  • Flexible – One kit for Extra- and Intra-Cellular cGMP quantification.
  • Convenient – Stable, 4 C liquid reagents.
  • Ready-to-Use – Complete kit with validated Cell Lysis Reagent Included.

Assay Principle

The aCella – cAMP Chemilumnescent Immunoassay kit is designed to quantitatively measure cAMP present in cell lysates, plasma, urine, saliva, tissue and culture media samples. The supplied Sample Diluent will lyse cells, stabilize cAMP and stop phopshodiesterease activity. A cAMP standard is provided to generate a standard curve for the assay. The supplied Plate Primer solution is added to the wells of a coated white microtiter plate, followed by standards or diluted samples. A cAMP-peroxidase conjugate is then added to the wells.

The binding reaction is initiated by the addition of a polyclonal antibody to cAMP. After a 2-hour incubation, the plate is washed and chemiluminescent substrate is added. The substrate immediately reacts with the bound cAMP-peroxidase conjugate. The generated chemiluminescent glow signal is measured. The concentration of the cAMP in the sample is calculated, after making correction for the dilution.

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References

  • Domek-Lopacinska, K. and Strosznajder, JB. “Cyclic GMP metabolism and its role in brain physiology “ (2005) J Physiol Pharmacol 56 Suppl 2, 15-34.
  • Lucas, K.A. et al. “Guanylyl cyclases and signaling by cyclic GMP “ (2000) Pharmacol Rev 52: 375-414.
  • Ashman, DF, et al., “Isolation of adenosine 3’, 5’-monophosphate and guanosine 3’, 5’-monophosphate from rat urine.“ (1963), Biochem Biophys Res Comm, 11: 330-4.
  • Potter LR, Abbey-Hosch S, and Dickey DM. “Natriuretic peptides, their receptors, and cyclic guanosine monophosphate-dependent signaling functions“. (2006) Endocr Rev 27: 47-72.
  • Waldman, SA and Murad, F., “Cyclic GMP synthesis and function “ (1987) Pharmacol Revs, 39: 163-197.
  • Tremblay J, Gerzer R, and Hamet P., “Cyclic GMP in cell function”. (1988) , Adv. 2nd Messanger & Phosphoprotein Res., 22: 319-383.
  • Matsumoto T, Kobayashi T, and Kamata K “Phosphodiesterases in the vascular system.” (2003) J Smooth Muscle Res 39: 67-86.

Kit contents and Long Term storage

ReagentPart#Temperature
Coater White Plastic Microfiber Plate (Coated with Donkey Anti-Donkey Anti-Sheep IgG)Part # 900012-8C
Cyclic GMP Standard, 1 Vial (70uL) at 640 pmol/mL in a Special Stabilizing SolutionPart # 900022-8C
Cyclic GMP Antibody, 1 Bottle (3mL) Mouse Monoclonal Antibody Specific for Cyclic GMPPart # 900032-8C
Cyclic GMP Conjugate, 1 Vial (150uL) cGMP Peroxidase Conjugate Concentrate in a Special Stabilizing SolutionPart # 900042-8C
Conjugate Diluent, 1 Bottle (3mL) Diluent Containing Special Stabilizers and AdditivesPart # 900052-8C
Plate Primer, 1 Bottle (5 mL) Neutralizing Solution Containing Special Stabilizers and AdditivesPart # 900072-8C
Acetic Anhydride, 1 Bottle (2mL)Part # 900082-8C
Triethylamine, 1 Bottle (4mL)Part # 900092-8C
Wash Buffer Concentrate, 1 Bottle (30mL) 20x Concentrate that must be diluted with deionized or distilled waterPart # 9000102-8C
Substrate Solution A, 1 bottle (6 mL)Part# 9000112-8C
Substrate Solution B, 1 bottle (6 mL)Part# 9000122-8C
Plate SealerPart# 900013
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