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aCella Cyclic AMP Cell Technology Ultra Sensitive Luminescence Kits
Cyclic AMP
aCella™ - cAMP: Cyclic AMP Chemiluminescent Immunoassay Kit

$345.00

in stock
Product code: CLACMP100
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Product Description

Key Benefits

  • Ultra-sensitive – Measures as little as < 1 fmol cAMP per sample.
  • Fast – Rapid assay, results in 2 hours.
  • Adaptable – Assay can be used with 96 or 384 – well plates.
  • Convenient – Suitable for use in multiple sample matices.

Assay Principle

The aCella – cAMP Chemilumnescent Immunoassay kit is designed to quantitatively measure cAMP present in cell lysates, plasma, urine, saliva, tissue and culture media samples. The supplied Sample Diluent will lyse cells, stabilize cAMP and stop phopshodiesterease activity. A cAMP standard is provided to generate a standard curve for the assay. The supplied Plate Primer solution is added to the wells of a coated white microtiter plate, followed by standards or diluted samples. A cAMP-peroxidase conjugate is then added to the wells.

The binding reaction is initiated by the addition of a polyclonal antibody to cAMP. After a 2-hour incubation, the plate is washed and chemiluminescent substrate is added. The substrate immediately reacts with the bound cAMP-peroxidase conjugate. The generated chemiluminescent glow signal is measured. The concentration of the cAMP in the sample is calculated, after making correction for the dilution.

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References

  • Sutherland, E. W. and Rall, T. W. Fractionation and Characterization of a Cyclic Adenine Ribonucleotide Formed by Tissue Particles. J. Biol. Chem., 232:1077, 1958.
  • Marsh, J.M., The Role of Cyclic AMP in Gonadal Steroidogenesis. Biol. Reprod, 14:30-53,1976.
  • Korenman, S.G. and Krall, J.F., The Role of Cyclic AMP in the Regulation of Smooth Muscle Cell Contraction in the Uterus. Biol. Reprod., 16:1-17, 1977.
  • Kelley, D.J., Bhattacharyya, A., Lahvis, G.P., Yin, J.C.P., Malter, J., and Davidson, R.J., The Cyclic AMP Phenotype of Fragile X and Autism. Neurosci. Biobehav. Rev., 32(8): 1533-1543, 2008.
  • http://www.hmdb.ca/metabolites/HMDB00058
  • Serezani, C.H., Ballinger, M.N., Aronoff, D.M., and Peters-Golden, M., Cyclic AMP. Master Regulator of Innate Immune Cell Function. Am. J. Resp. Cell and Mol. Biol., 39 (2): 127, 2008.
  • Hannila, S.S., and Filbin, M.T., The role of cyclic AMP signaling in promoting axonal regeneration after spinal cord injury. Exp. Neurol., 209(2): 321–332, 2008.
  • Shankar, D.B, Cheng, J.C., and Sakamoto, K.M., Role of cyclic AMP response element binding protein in human leukemias. Cancer, 104(9): 1819-24, 2005.
  • Galea E. and Feinstein, D.L., Regulation of the expression of the inflammatory nitric oxide synthase (NOS2) by cyclic AMP. FASEB J., 13:2125-2137, 1999.
  • NIH Clinical Center, http://cclnprod.cc.nih.gov/dlm/testguide.nsf/Index/EB6E90F8D951346F85256B A4004C96E4?OpenDocument
  • NIH Clinical Center, http://cclnprod.cc.nih.gov/dlm/testguide.nsf/Index/24B381AEE513EB8785256B A40052ADAD?OpenDocument
  • Sproles, A.C., Cyclic AMP Concentration in Saliva of Normal Children and Children with Down’s Syndrome, J. Dent. Res., 1976, 52, 915-917.

Kit contents and Long Term storage

ReagentPart#Temperature
Coater White Plastic Microfiber Plate (Coated with Donkey Anti-Sheep IgG)Part # 900142-8C
Cyclic AMP Standard, 1 Vial (125uL) cAMP at 1,500 pmol/mL in a Special Stabilizing SolutionPart # 900152-8C
Cyclic AMP Antibody, 1 Bottle (3mL) Sheep Antibody Specific for Cyclic AMPPart # 900162-8C
Cyclic AMP Conjugate, 1 Vial 50X cAMP Peroxidase Conjugate Concentrate Stock in a Special Stabilizing SolutionPart # 900172-8C
Conjugate Diluent, 1 Bottle (3mL) Diluent Containing Special Stabilizers and AdditivesPart # 900182-8C
Sample Diluent, 1 Bottle (28mL) Diluent Containing Special Stabilizers and Additives. Ready to UsePart# 900192-8C
Plate Primer, 1 Bottle (5 mL) Neutralizing Solution Containing Special Stabilizers and AdditivesPart # 900202-8C
Acetic Anhydride, 1 Bottle (2mL)Part # 900212-8C
Triethylamine, 1 Bottle (4mL)Part # 900222-8C
Wash Buffer Concentrate, 1 Bottle (30mL) 20x Concentrate that must be diluted with deionized or distilled waterPart # 900232-8C
Substrate Solution A, 1 bottle (6 mL)Part# 900242-8C
Substrate Solution B, 1 bottle (6 mL)Part# 900252-8C
Plate SealerPart# 90020
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