$185.00 – $550.00
- Additional information
- ASSAY PRINCIPLE
- Readable Documents
- Kit contents and storage
- Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition.
- Cell permeablity permits direct visualization of cytosolic apoptotic events.
- Apoptotic cell population does not diminish over time.
- Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.
- Works in diverse cell lines: human, rodent, Drosophila.
- Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells.
- Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.
- Applications – Works with fluorescence microscope, 96-well fluorescence plate readers
- Yields both quantitative and qualitative results. Gives strong signal with little background noise.
APO LOGIX SR kits contain a generic sulforhodamine labeled caspase inhibitor (sulforhodamine-peptide-fluoromethyl ketone). This reagent is cell permeable and is used on whole cells to detect apoptosis. Apoptotic cells are detected by a fluorescence plate reader or fluorescence microscope using an excitation source at 550nm and measuring emission at 595nm. The assay takes about 1 hr to complete
APO LOGIX Sulforhodamine
Jurkat cells stimulated with staurosporine for 2 hours and then labeled with SR-VAD-FMK.
Left side: 30X phase contrast
Right side: 30X fluorescence microscope. Excitation: 550nm emission > 580nm.
APO LOGIX Sulforhodamine
Jurkat cells stimulated with staurosporine for 2 hours. Cells were then stained with SR-VAD-FMK for 1 hour and read in a 96 well fluorescence plate reader.
Kit contents and storage
|Part # 679||Lyophilized SR-VAD-FMK||2-8°C|
|Part # 635||10X Wash Buffer||2-8°C|
|Part # 636||10X Fixitive||2-8°C|
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