Key Benefits

  • Homogenous assay for active caspase 3/7.
  • Breakthrough in cell lysis buffer and preservation of caspase activity.
  • Results in a no-wash, one-step assay.
  • No need to wash out media from cell samples, just add the reagent directly to your experimental samples.
  • Easy to Use: No need to make cell lysates or run Western blots.
  • Works with suspension and adherent cells.

Additional information

Kit Size

100, 500, 1000


<p style=”font-weight: 500; color: #666666;”>Cell Technology’s APO 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The absorption and emission properties of the R110 dye are suppressed when attached to the z-DEVD peptide sequence. When R110 is cleaved away, by active caspase3/7, form the quenching DEVD sequence, the free dye excites at 488nm and emits at 515-530 nm. As a result of a novel and proprietary Lysis Buffer System, the APO 3/7 HTS Assay is a homogenous platform that can be utilized for high throughput fluorescence plate reader applications. The reagent is directly added to the samples thus eliminating any wash steps.</p>
<p style=”font-weight: 500; color: #666666;”><strong style=”font-weight: bold;”>Figure.</strong> In this figure, Jurkat cells were stimulated with various concentrations of staurosporine for 3 hours, after which caspase 3/7 activity was analyzed using the APO 3/7 HTS kit.</p>

Readable Documents

Title Name
Protocol Protocol.pdf
Datasheet Datasheet.pdf
msds msds.pdf

Kit contents and storage

Part# Reagent Temperature
Part# 4004 Caspase 3/7 Reagent (z-DEVD) 2 Rodamine 110, 1 Vial -20°C
Part# 3005 Cell Lysis Buffer, 1 Bottle 2-8°C



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