Apo Active 3PE

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Key Benefits

  • Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
  • Applications – Flow Cytometery, Fluorescent Microscopy, or Fluorescent plate reader.
  • Yields both quantitative and qualitative results. Gives a strong positive signal.
  • Can be used in conjunction with other antibodies or stains.
  • No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
  • Works with human, mouse and rat cell lines.

Additional information

Kit Size

25, 100

phycoerythrin_antibody_based_caspase1Cell Technology’s APO-ACTIVE 3 PE Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary PE labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal. PE labeling allows for use with a variety of applications.


(A)

Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.

phycoerythrin_antibody_based_caspase2(B)
Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.

TitleFileLinkAuthor(s)JournalYear; Edition:Pages
The endoplasmic reticulum is the site of cholestrol-induced cytotoxicity in macrophageshttp://www.cumc.columbia.edu/dept/medicine/tabas_site/pubs/UPR-NatureCB.pdfFeng, Yao, Li, Devlin et al -Nature Cell BiologyVol 5, No 9, September 2003, pp 781-792
Combination chemotherapy with gemcitabine and biotherapy with opioid growth factor (OGF) enhances the growth inhibition of pancreatic adenocarcinomahttp://www.springerlink.com/content/h025524175r45553/Ian S. Zagon, Jeffrey R. Jaglowski, et.al.Cancer Chemotherapy and PharmacologyVol 56/ No 5, Nov 2005
The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cellshttp://www3.interscience.wiley.com/journal/119420124/abstract?CRETRY=1&SRETRY=0Sasha H. Shafikhani, Christina Morales and Joanne EngelCellular MicrobiologyVol 10 Issue 4, pp 994-1007, Dec 2007
Monoclonal Anti–Interleukin 23 Reverses Active Colitis in a T Cell–Mediated Model in Micehttp://linkinghub.elsevier.com/retrieve/pii/S0016508507006300C. Elson, Y. Cong, C. Weaver, T. Schoeb, T. McClanahan, R. Fick, R. KasteleinGastroenterologyVolume 132, Issue 7, Pages 2359-2370 (2007)
Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injuryhttp://ajprenal.physiology.org/cgi/content/abstract/00452.2006v1Markus Horbelt, So-Young Lee, Henry E Mang, Nicole L. Knipe, Yoshikazu Sado, Andreas Kribben, and Timothy A SuttonAm J Physiol Renal Physiol(July 11, 2007)
Cytotoxicity and Secretion of Gamma Interferon Are Carried Out by Distinct CD8 T Cells during Mycobacterium tuberculosis Infectionhttp://iai.asm.org/cgi/content/abstract/77/10/4621Thorbjorg Einarsdottir, Euan Lockhart, and JoAnne L. FlynnAmerican Society for Microbiology - Infection and ImmunityOctober 2009, p. 4621-4630, Vol. 77, No. 10.
Converting cell lines representing hematological malignancies from glucocorticoid-resistant to glucocorticoid-sensitive: Signaling pathway interactionshttp://www.lrjournal.com/article/S0145-2126%2808%2900462-1/abstractAnna S. Garza, Aaron L. Miller, Betty H. Johnson, E. Brad ThompsonLeukemia ResearchVol 33, Issue 5, pp 717-727. May 2009
Reference
Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial Killers: orderingcaspase activation events in apoptosis. Cell Death and Differ. 6:1067-1074.
Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang, N. J. Bump, C. R.Ferenz, S. Franklin, T. Ghayur, M. C. Hackett and L. D. Hammill. 1994. Crystal Structure of the Cysteine Protease Interleukin-1ß-Converting Enzyme: A(p20/p10)2 Homodimer. Cell 78:343-352.
Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M. A.Navia, M. A. Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D. J.Livingston. 1994. Structure and mechanism of interleukin-1 beta converting enzyme. Nature 370: 270-275.
Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M. Labelle,E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A. Thornberry and J.W. Becker. 1996. The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3(7): 619-625.
Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060-1066.
Alnemri, E.S. et al (1996) Cell 87:171
Trends Biochem Sci 22,388 (1997)
Nature 376, 37 (1995).
Part#ReagentTemperature
Part# 1002Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial2-8C
Part# 2004PE Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial2-8C
Part# 300110X FixativeRoom Temperature

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