Apo Active 3PE (PAB200)
- Additional information
- Assay Principle
- Kit Contents Long Term Storage
- Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
- Applications – Flow Cytometery, Fluorescent Microscopy, or Fluorescent plate reader.
- Yields both quantitative and qualitative results. Gives a strong positive signal.
- Can be used in conjunction with other antibodies or stains.
- No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
- Works with human, mouse and rat cell lines.
Cell Technology’s APO-ACTIVE 3 PE Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary PE labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal. PE labeling allows for use with a variety of applications.
Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.
Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.
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- Alnemri, E.S. et al (1996) Cell 87:171
- Trends Biochem Sci 22,388 (1997)
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- Part# 1002: Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial (2-8C)
- Part# 2004: PE Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial (2-8C)
- Part# 3001: 10X Fixative (Room Temperature)