Apo Active 3PE (PAB200)

$470.00

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Description

Key Benefits

  • Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
  • Applications – Flow Cytometery, Fluorescent Microscopy, or Fluorescent plate reader.
  • Yields both quantitative and qualitative results. Gives a strong positive signal.
  • Can be used in conjunction with other antibodies or stains.
  • No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
  • Works with human, mouse and rat cell lines.

Additional information

Kit Size

25, 100

phycoerythrin_antibody_based_caspase1Cell Technology’s APO-ACTIVE 3 PE Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary PE labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal. PE labeling allows for use with a variety of applications.


(A)

Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.

phycoerythrin_antibody_based_caspase2(B)
Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.

    The endoplasmic reticulum is the site of cholestrol-induced cytotoxicity in macrophagesCombination chemotherapy with gemcitabine and biotherapy with opioid growth factor (OGF) enhances the growth inhibition of pancreatic adenocarcinomaThe Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cellsMonoclonal Anti–Interleukin 23 Reverses Active Colitis in a T Cell–Mediated Model in MiceAcute and chronic microvascular alterations in a mouse model of ischemic acute kidney injuryCytotoxicity and Secretion of Gamma Interferon Are Carried Out by Distinct CD8 T Cells during Mycobacterium tuberculosis InfectionConverting cell lines representing hematological malignancies from glucocorticoid-resistant to glucocorticoid-sensitive: Signaling pathway interactions
  • Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial Killers: orderingcaspase activation events in apoptosis. Cell Death and Differ. 6:1067-1074.
  • Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang, N. J. Bump, C. R.Ferenz, S. Franklin, T. Ghayur, M. C. Hackett and L. D. Hammill. 1994. Crystal Structure of the Cysteine Protease Interleukin-1ß-Converting Enzyme: A(p20/p10)2 Homodimer. Cell 78:343-352.
  • Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M. A.Navia, M. A. Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D. J.Livingston. 1994. Structure and mechanism of interleukin-1 beta converting enzyme. Nature 370: 270-275.
  • Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M. Labelle,E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A. Thornberry and J.W. Becker. 1996. The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3(7): 619-625.
  • Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060-1066.
  • Alnemri, E.S. et al (1996) Cell 87:171
  • Trends Biochem Sci 22,388 (1997)
  • Nature 376, 37 (1995).
  • Part# 1002: Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial (2-8C)
  • Part# 2004: PE Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial (2-8C)
  • Part# 3001: 10X Fixative (Room Temperature)