Apo Active 3 FITC




Key Benefits

  • Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
  • Applications – Works with a fluoresence microscope, 96 well plate reader, or flow cytometer.
  • Yields both quantitative and qualitative results. Gives a strong positive signal.
  • Can be used in conjunction with other antibodies or stains.
  • No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
  • Works with human, mouse and rat cell lines.

Additional information

Kit Size

25, 100



Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.

Fig (A). Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.


Fig (B). Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody.

Readable Documents

Title Name
Protocol Protocol.pdf
Datasheet Datasheet.pdf
msds msds.pdf

Kit contents and storage

Part# Reagent Temperature
Part # 1002 Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial 2-8°C
Part # 2002 FITC Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial 2-8°C
Part # 3001 10X Fixative, 1 Vial 2-8°C



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Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060-1066.
Alnemri, E.S. et al (1996) Cell 87:171
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Srinivasan A, et al Cell Death and Differentiation (1998) 5: 1004-1016


Title File Link Author(s) Journal Year; Edition:Pages
The endoplasmic reticulum is the site of cholestrol-induced cytotoxicity in macrophages http://www.cumc.columbia.edu/dept/medicine/tabas_site/pubs/UPR-NatureCB.pdf Feng, Yao, Li, Devlin et al Nature Cell Biology Vol 5, No 9 September 2003, pp 781-792
Combination chemotherapy with gemcitabine and biotherapy with opioid growth factor (OGF) enhances the growth inhibition of pancreatic adenocarcinoma http://www.springerlink.com/content/h025524175r45553/ Ian S. Zagon, Jeffrey R. Jaglowski, et.al. – Cancer Chemotherapy and Pharmacology Vol 56/ No 5, Nov 2005
The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cells http://www3.interscience.wiley.com/journal/119420124/abstract?CRETRY=1&SRETRY=0 Sasha H. Shafikhani, Christina Morales and Joanne Engel Cellular Microbiology Vol 10 Issue 4, pp 994-1007, Dec 2007
Monoclonal Anti–Interleukin 23 Reverses Active Colitis in a T Cell–Mediated Model in Mice http://linkinghub.elsevier.com/retrieve/pii/S0016508507006300 C. Elson, Y. Cong, C. Weaver, T. Schoeb, T. McClanahan, R. Fick, R. Kastelein Gastroenterology Volume 132, Issue 7, Pages 2359-2370 (2007)
Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injury http://ajprenal.physiology.org/cgi/content/abstract/00452.2006v1 Markus Horbelt, So-Young Lee, Henry E Mang, Nicole L. Knipe, Yoshikazu Sado, Andreas Kribben, and Timothy A Sutton Am J Physiol Renal Physiol July 11, 2007
Cytotoxicity and Secretion of Gamma Interferon Are Carried Out by Distinct CD8 T Cells during Mycobacterium tuberculosis Infection http://iai.asm.org/cgi/content/abstract/77/10/4621 Thorbjorg Einarsdottir, Euan Lockhart, and JoAnne L. Flynn American Society for Microbiology – Infection and Immunity October 2009, p. 4621-4630, Vol. 77, No. 10.
Converting cell lines representing hematological malignancies from glucocorticoid-resistant to glucocorticoid-sensitive: Signaling pathway interactions http://www.lrjournal.com/article/S0145-2126%2808%2900462-1/abstract Anna S. Garza, Aaron L. Miller, Betty H. Johnson, E. Brad Thompson Leukemia Research Vol Vol 33, Issue 5, pp 717-727. May 2009