Apo Active 3 FITC

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Key Benefits

  • Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
  • Applications – Works with a fluoresence microscope, 96 well plate reader, or flow cytometer.
  • Yields both quantitative and qualitative results. Gives a strong positive signal.
  • Can be used in conjunction with other antibodies or stains.
  • No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
  • Works with human, mouse and rat cell lines.

Additional information

Kit Size

25, 100

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Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.

Fig (A). Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.

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Fig (B). Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody.

TitleFileLinkAuthor(s)JournalYear; Edition:Pages
The endoplasmic reticulum is the site of cholestrol-induced cytotoxicity in macrophageshttp://www.cumc.columbia.edu/dept/medicine/tabas_site/pubs/UPR-NatureCB.pdfFeng, Yao, Li, Devlin et alNature Cell Biology Vol 5, No 9September 2003, pp 781-792
Combination chemotherapy with gemcitabine and biotherapy with opioid growth factor (OGF) enhances the growth inhibition of pancreatic adenocarcinomahttp://www.springerlink.com/content/h025524175r45553/Ian S. Zagon, Jeffrey R. Jaglowski, et.al. -Cancer Chemotherapy and PharmacologyVol 56/ No 5, Nov 2005
The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cellshttp://www3.interscience.wiley.com/journal/119420124/abstract?CRETRY=1&SRETRY=0Sasha H. Shafikhani, Christina Morales and Joanne EngelCellular MicrobiologyVol 10 Issue 4, pp 994-1007, Dec 2007
Monoclonal Anti–Interleukin 23 Reverses Active Colitis in a T Cell–Mediated Model in Micehttp://linkinghub.elsevier.com/retrieve/pii/S0016508507006300C. Elson, Y. Cong, C. Weaver, T. Schoeb, T. McClanahan, R. Fick, R. KasteleinGastroenterologyVolume 132, Issue 7, Pages 2359-2370 (2007)
Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injuryhttp://ajprenal.physiology.org/cgi/content/abstract/00452.2006v1Markus Horbelt, So-Young Lee, Henry E Mang, Nicole L. Knipe, Yoshikazu Sado, Andreas Kribben, and Timothy A SuttonAm J Physiol Renal PhysiolJuly 11, 2007
Cytotoxicity and Secretion of Gamma Interferon Are Carried Out by Distinct CD8 T Cells during Mycobacterium tuberculosis Infectionhttp://iai.asm.org/cgi/content/abstract/77/10/4621Thorbjorg Einarsdottir, Euan Lockhart, and JoAnne L. FlynnAmerican Society for Microbiology - Infection and ImmunityOctober 2009, p. 4621-4630, Vol. 77, No. 10.
Converting cell lines representing hematological malignancies from glucocorticoid-resistant to glucocorticoid-sensitive: Signaling pathway interactionshttp://www.lrjournal.com/article/S0145-2126%2808%2900462-1/abstractAnna S. Garza, Aaron L. Miller, Betty H. Johnson, E. Brad ThompsonLeukemia Research VolVol 33, Issue 5, pp 717-727. May 2009
Reference
Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial Killers: ordering caspase activation events in apoptosis. Cell Death and Differ. 6:1067-1074
Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang, N. J. Bump, C. R. Ferenz, S. Franklin, T. Ghayur, M. C. Hackett and L. D. Hammill. 1994. Crystal Structure of the Cysteine Protease Interleukin-1ß-Converting Enzyme: A (p20/p10)2 Homodimer. Cell 78:343-352.
Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M. A. Navia, M. A. Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D. J. Livingston. 1994. Structure and mechanism of interleukin-1 beta converting enzyme. Nature 370: 270-275.
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Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060-1066.
Alnemri, E.S. et al (1996) Cell 87:171
Trends Biochem Sci 22,388 (1997)
Nature 376, 37 (1995).
Srinivasan A, et al Cell Death and Differentiation (1998) 5: 1004-1016
Part#ReagentTemperature
Part # 1002Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial2-8°C
Part # 2002FITC Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial2-8°C
Part # 300110X Fixative, 1 Vial2-8°C

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