Apo Active 3 FITC
$155.00 – $445.00
- Description
- Additional information
- ASSAY PRINCIPLE
- Readable Documents
- Kit contents and storage
- References
- Citations
Description
Key Benefits
- Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
- Applications – Works with a fluoresence microscope, 96 well plate reader, or flow cytometer.
- Yields both quantitative and qualitative results. Gives a strong positive signal.
- Can be used in conjunction with other antibodies or stains.
- No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
- Works with human, mouse and rat cell lines.
Additional information
| Kit Size | 25, 100 |
|---|
ASSAY PRINCIPLE
Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.
Fig (A). Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.
Fig (B). Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody.
Readable Documents
| Title | Name |
|---|---|
| Protocol | Protocol.pdf |
| Datasheet | Datasheet.pdf |
| msds | msds.pdf |
Kit contents and storage
| Part# | Reagent | Temperature |
| Part # 1002 | Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial | 2-8°C |
| Part # 2002 | FITC Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial | 2-8°C |
| Part # 3001 | 10X Fixative, 1 Vial | 2-8°C |
References
| Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial Killers: ordering caspase activation events in apoptosis. Cell Death and Differ. 6:1067-1074 |
| Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang, N. J. Bump, C. R. Ferenz, S. Franklin, T. Ghayur, M. C. Hackett and L. D. Hammill. 1994. Crystal Structure of the Cysteine Protease Interleukin-1ß-Converting Enzyme: A (p20/p10)2 Homodimer. Cell 78:343-352. |
| Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M. A. Navia, M. A. Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D. J. Livingston. 1994. Structure and mechanism of interleukin-1 beta converting enzyme. Nature 370: 270-275. |
| Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M. Labelle, E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A. Thornberry and J. W. Becker. 1996. The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3(7): 619-625. |
| Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060-1066. |
| Alnemri, E.S. et al (1996) Cell 87:171 |
| Trends Biochem Sci 22,388 (1997) |
| Nature 376, 37 (1995). |
| Srinivasan A, et al Cell Death and Differentiation (1998) 5: 1004-1016 |
Citations
| Title | File | Link | Author(s) | Journal | Year; Edition:Pages |
| The endoplasmic reticulum is the site of cholestrol-induced cytotoxicity in macrophages | http://www.cumc.columbia.edu/dept/medicine/tabas_site/pubs/UPR-NatureCB.pdf | Feng, Yao, Li, Devlin et al | Nature Cell Biology Vol 5, No 9 | September 2003, pp 781-792 | |
| Combination chemotherapy with gemcitabine and biotherapy with opioid growth factor (OGF) enhances the growth inhibition of pancreatic adenocarcinoma | http://www.springerlink.com/content/h025524175r45553/ | Ian S. Zagon, Jeffrey R. Jaglowski, et.al. – | Cancer Chemotherapy and Pharmacology | Vol 56/ No 5, Nov 2005 | |
| The Pseudomonas aeruginosa type III secreted toxin ExoT is necessary and sufficient to induce apoptosis in epithelial cells | http://www3.interscience.wiley.com/journal/119420124/abstract?CRETRY=1&SRETRY=0 | Sasha H. Shafikhani, Christina Morales and Joanne Engel | Cellular Microbiology | Vol 10 Issue 4, pp 994-1007, Dec 2007 | |
| Monoclonal Anti–Interleukin 23 Reverses Active Colitis in a T Cell–Mediated Model in Mice | http://linkinghub.elsevier.com/retrieve/pii/S0016508507006300 | C. Elson, Y. Cong, C. Weaver, T. Schoeb, T. McClanahan, R. Fick, R. Kastelein | Gastroenterology | Volume 132, Issue 7, Pages 2359-2370 (2007) | |
| Acute and chronic microvascular alterations in a mouse model of ischemic acute kidney injury | http://ajprenal.physiology.org/cgi/content/abstract/00452.2006v1 | Markus Horbelt, So-Young Lee, Henry E Mang, Nicole L. Knipe, Yoshikazu Sado, Andreas Kribben, and Timothy A Sutton | Am J Physiol Renal Physiol | July 11, 2007 | |
| Cytotoxicity and Secretion of Gamma Interferon Are Carried Out by Distinct CD8 T Cells during Mycobacterium tuberculosis Infection | http://iai.asm.org/cgi/content/abstract/77/10/4621 | Thorbjorg Einarsdottir, Euan Lockhart, and JoAnne L. Flynn | American Society for Microbiology – Infection and Immunity | October 2009, p. 4621-4630, Vol. 77, No. 10. | |
| Converting cell lines representing hematological malignancies from glucocorticoid-resistant to glucocorticoid-sensitive: Signaling pathway interactions | http://www.lrjournal.com/article/S0145-2126%2808%2900462-1/abstract | Anna S. Garza, Aaron L. Miller, Betty H. Johnson, E. Brad Thompson | Leukemia Research Vol | Vol 33, Issue 5, pp 717-727. May 2009 |
