Apo Active 3 FITC (FAB200)
- Additional information
- Assay Principle
- Readable Documents
- Kit Contents Long Term Storage
- Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
- Applications – Works with a fluorescence microscope, 96 well plate reader, or flow cytometer.
- Yields both quantitative and qualitative results. Gives a strong positive signal.
- Can be used in conjunction with other antibodies or stains.
- No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
- Works with human, mouse and rat cell lines.
Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.
Fig (A). Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.
Fig (B). Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody.
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- Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M. Labelle, E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A. Thornberry and J. W. Becker. 1996. The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3(7): 619-625.
- Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060-1066.
- Alnemri, E.S. et al (1996) Cell 87:171
- Trends Biochem Sci 22,388 (1997)
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- Srinivasan A, et al Cell Death and Differentiation (1998) 5: 1004-1016
- Part # 1002: Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial (2-8°C)
- Part # 2002: FITC Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial (2-8°C)
- Part # 3001: 10X Fixative, 1 Vial (2-8°C)