Apo Active 3 FITC
$155.00 – $445.00
- Additional information
- ASSAY PRINCIPLE
- Readable Documents
- Kit contents and storage
- Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.
- Applications – Works with a fluoresence microscope, 96 well plate reader, or flow cytometer.
- Yields both quantitative and qualitative results. Gives a strong positive signal.
- Can be used in conjunction with other antibodies or stains.
- No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.
- Works with human, mouse and rat cell lines.
Cell Technology ’s APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary FITC labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal.
Fig (A). Jurkat Cells were stimulated with DMSO for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with PE labeled Goat anti Rabbit antibody.
Fig (B). Jurkat Cells were stimulated with staurosporine for 2 hours. The cells were washed and fixed for 15 minutes. The cells were then permeabilized with saponin and stained with Rabbit anti active caspase 3 antibody for 1 hour. The samples were then stained with FITC labeled Goat anti Rabbit antibody.
Kit contents and storage
|Part # 1002||Rabbit Anti Cleaved Caspase 3 Affinity Purified Polyclonal Antibody, 1 Vial||2-8°C|
|Part # 2002||FITC Labeled Goat Anti Rabbit Affinity Purified Polyclonal Antibody, 1 Vial||2-8°C|
|Part # 3001||10X Fixative, 1 Vial||2-8°C|
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