- Reduces the effects of the sample matrix and variation among samples. Pre-dilution of the samples is not necessary.
- Reduces background noise caused by nonspecific interactions between the sample matrix proteins and the plate surface.
Just pipette diluent directly onto the plate before you add samples.
A buffer is used to equalize any differences between the sample matrix (serum, plasma, urine, cell culture fluid) and the diluent is used to generate the standard curve.
EAD100 General Assay Diluent for Serum & Plasma Samples (Sandwich Format) EAD200 IgM (Positive Interference) Reducing Assay Diluent (All ELISAs) EAD300 Nonmammalian-Based Assay Diluent (Ag Down and Sandwich ELISAs) EAD400 Antigen-Down Assay Diluent (Ag Down and Sandwich ELISAs)
Cell Technology’s Antigen-Down Assay Diluent is specifically formulated for testing plasma and serum samples in antigen-down ELISA formats. Proprietary additives, including EDTA, have been incorporated into this diluent that eliminate the risk of plasma sample clots in the ELISA plate well while also minimizing nonspecific binding interactions associated with these complex matrices.