$365.00 – $1,530.00
Commercially available ADP is contaminated with trace amounts of ATP. In bioluminescence assays this trace ATP contamination results in high backgrounds and reduces assay sensitivity. Cell Technology’s ADP is purified to remove these trace ATP contaminants and as a result produces ADP that is greater than 99.9% pure.
Molecular weight: 427.20
Molecular formula: C10H15N5O10P2
>99.9% Pure ADP as tested by P31 NMR – Virtually Free of all ATP contaminants.
This ADP is designed for use in bioluminescence applications where minimal background ATP contamination is required. ADP can be used as a substrate in enzymatic reactions to generate ATP. Subsequently ATP can be detected via the bioluminescence reaction of luciferase/luciferin.
Fig. shows the two phosphate moieties of ADP, with no trace of a peak indicating the third phosphate of ATP using P31 NMR.
Supplied as a 10mM (approx) solution buffered in 60mM TRIS at pH 7.0
Precautions and Storage
- Avoid repeated freeze/thaw cycles by freezing down as aliquots.
- Product should be stored < -20°C