5x Antigen

Improves Assays

• Increases the Sensitivity of the Assay.
• Decreases Backgrounds.
• Stabilizes Newly Conjugated Proteins. Also reconstitutes lyophilized conjugates and diluents concentrates conjugates.
• Prolongs the Shelf-Life of HRP-conjugated proteins and enhancing their utility in ELISA applications.
• Extends the Useful Titer of the conjugate.
• Conserves Valuable Reagents. By promoting a higher specific signal, less conjugate may be needed to generate a signal when a stabilizing conjugate diluent is used.
• Lengthens the Shelf-Life of conjugated antibodies for future use. With a longer shelf-life, conjugates may be prepared in batches for use in future plate coating runs. Using conjugates from the lot generally reduces inter-assay variability and increases consistency.

Directions

To stabilize newly conjugate proteins, add 1 part of the appropriate 5x conjugate diluent (that has not been diluted to 1x) to 4 parts of the new conjugate solution.
To reconstitute lyophilized conjugates, simply pipette a known amount of a 1x conjugate diluent into the vial. The liquid conjugate is now ready to be used directly, or further diluted to the required concentration.
To dilute concentrated conjugates for use in an assay, pipette a known amount of the conjugate stock into a 1x conjugate diluent.

How it Works

Conjugate Diluents stabilize newly conjugated proteins, reconstitute yophilized conjugates, and dilute concentrated conjugates. Cell Technology’s conjugate diluents stabilize the immunoglobulin and HRP components of the conjugate – protein complex. All conjugate diluents are available at 1x and 5x concentrations. When stored unopened and protected from light, a shelf-life of 18 months can be expected at 2°-8°C.

ECD200 Antigen-Down Conjugate Diluent & Stabilizer

Antigen-Down Conjugate Diluent & Stabilizer is a unique formulation specifically designed for use with anti-IgG-HRP conjugates. This diluent contains proprietary non-mammalian protein additives which will not interfere with anti-IgG-HRP conjugates used in antigen-down and sandwich ELISA formats. These additives also provide additional structural stability to the IgG-HRP conjugate protein complex. CD2 will also inhibit conjugate binding to nonspecific serum proteins bound to the plate, thus reducing conjugate bridging on the plate surface and lowering background noise. Antimicrobial agents have been added to retard bacterial growth. When stored unopened and protected from light, a shelf-life of 18 months can be expected at 2°-8°C.