Cell Technology possesses a customizable ADCC/CMC
assay platform that facilitates the selection of
antibody drug candidates. Our bioluminescent ultra
sensitive
aCella-TOX
Assay, is rapidly becoming the standard for ADCC/CMC
assays within the therapeutics industry.
We provide ADCC assay services to determine the
activity of the antibody against specified target cell
lines. The result would be, a dose dependent kinetic
response of the therapeutic antibody. The assay
service includes optimization of E/T ratios,
measurement of ADCC activity at a series of antibody
concentrations, at a certain E:T ratio. Correlation
data with FACS analysis can be generated upon request.
Steps Involved for the ADCC implementation
1.
Determination of optimal E:T ratios for the
Target Cell line.
2.
Procurement of the Antibody (or Antibodies) and
Target Cell line (s) to be tested.
3.
A dose response curve would be generated
using aCella-TOX or FACS analysis or both. (as
requested).
Supplies and information to be provided by the
client
1.
Target Cell line(s) (adherent or suspension)
(frozen vial(s)) and sufficient Antibody for the
project.
2.
Cell Culture Media and conditions for growth of
the target cell line
3.
Antibody concentrations (if
known)
4.
Number of PBMC donors to be tested.
5.
Information on Antigen expression Level on the
target cell line (s) (FACS analysis can be done at the
request of the client to determine this).
Supplies to be provided by Cell Technology
All additional supplies would be procured by Cell
Technology, and billed to the client at cost.
Final Report would be turned in after the Project
with a dose response curve, showing Antibody
dependence.
Typical time frame is
approximately 4 weeks / Cell line / Antibody.
For Schedule and Pricing, please
call 888-727-7297 or 650-960-2170, or email us at sales@technology.com.
