Contract Research Services


Cell Based Assays

Antibody Dependent Cellular Cytotoxicity (ADCC), & Cell Mediated Cytotoxicity (CMC)

Cell Technology possesses a customizable ADCC/CMC assay platform that facilitates the selection of antibody drug candidates. Our bioluminescent ultra sensitive aCella-TOX Assay, is rapidly becoming the standard for ADCC/CMC assays within the therapeutics industry.

We provide ADCC assay services to determine the activity of the antibody against specified target cell lines. The result would be, a dose dependent kinetic response of the therapeutic antibody. The assay service includes optimization of E/T ratios, measurement of ADCC activity at a series of antibody concentrations, at a certain E:T ratio. Correlation data with FACS analysis can be generated upon request.
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Figure: 5000 Ramos cells/well were incubated with serially diluted Rituxan antibody for 15 minutes prior to the addition of purified NK cells stimulated overnight with IL-2 . The ADCC reaction was further incubated for 2 hours (top graph) or 3.5 hours (bottom graph) at the specified E:T ratios. % Cytotoxicity was measured using the aCellaTOX assay. 3.5hour timepoint has been optimized with the 10:1 E:T ratio (Blue). Graph with 20:1 E:T ratio (Red) is flat.(100% cytotoxicity)

Steps Involved for the ADCC implementation

  1. Determination of optimal E:T ratios for the Target Cell line.
  2. Procurement of the Antibody (or Antibodies) and Target Cell line (s) to be tested.
  3. A dose response curve would be generated using aCella-TOX or FACS analysis or both. (as requested).


Supplies and information to be provided by the client

  1. Target Cell line(s) (adherent or suspension) (frozen vial(s)) and sufficient Antibody for the project.
  2. Cell Culture Media and conditions for growth of the target cell line
  3. Antibody concentrations (if known)
  4. Number of PBMC donors to be tested.
  5. Information on Antigen expression Level on the target cell line (s) (FACS analysis can be done at the request of the client to determine this).


Supplies to be provided by Cell Technology

All additional supplies would be procured by Cell Technology, and billed to the client at cost.

Final Report would be turned in after the Project with a dose response curve, showing Antibody dependence.

Typical time frame is approximately 4 weeks / Cell line / Antibody.

For Schedule and Pricing, please call 888-727-7297 or 650-960-2170, or email us at sales@technology.com.