Our Coating Buffer is an optimal formulation of buffers and salts which maximizes the adsorption of antibodies and antigens onto polystyrene plates. During the adsorption process, the Coating Buffer stabilizes coated proteins by maintaining their tertiary three-dimensional structure. By stabilizing the adsorbed protein, antigenic regions are preserved, allowing for greater binding reactivity with the detection molecule, thereby enhancing the specific signal. By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents.

Simply dilute Coating Buffer 1:5 (for example, add 100mL Coating Buffer to 400mL diH20 for a final volume of 500mL) , add your protein antigen or antibody, let the solution stir for 15 minutes, and pipette onto the plate.

Cell Technology has specifically formulated a unique plate coating buffer for use with antibodies and antigens. The pH and ionic strength of this buffer have been specifically formulated to create an optimal coating environment which maximizes the adsorption efficiency of most antibodies and protein antigens onto polystyrene plate surfaces. This buffer also stabilizes coated proteins, preserving antigenic regions, and allowing for greater binding reactivity with the detection molecule, enhancing the specific signal. By generating a higher specific signal, a lower concentration of coat protein may be needed when this buffer is used, thereby saving valuable reagents.