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| Caspase / Apoptosis
Detection |
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High Throughput Screening
- Apo3HTS |
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Caspase Poly,1,2,3,7,8,9,10
Detection - FAM-FMK |
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Caspase Poly, 1,2,3,8,9,10,
Detection
- (SR-FMK) |
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Antibody Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
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Antibody Specific Caspase 3 Detection
- Apo Active 3 (PE) |
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| NOS/ROS Detection Kits |
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| Fluorescent Enzymatic
Assays |
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| Mitochondria
Membrane Potential detection |
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| aCella - Bioluminescence
Assays Kits |
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| Cytotoxicity |
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| ELISA
Products |
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| Cathepsin B,K,L
Detection Kits |
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APO ACTIVE 3 Phycoerythrin (PE) ™
Antibody Specific
Active Caspase 3 Detection Kit
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| Key Benefits: |
-
Highly specific for
active human and murine caspase 3. Other assays require the utilization
of peptide based (DEVD) reagents that tend to cross-react with caspase
7 and other caspases.
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Applications -
Works with a flow cytometer, flourescence microscope, or 96 well plate
reader.
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Yields both
quantitative and qualitative results. Gives a strong positive signal.
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Can be used in
conjunction with other antibodies or stains.
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No need to make cell
lysates or run western blots. Cells can be fixed and analyzed later.
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Works with human,
mouse and rat cell lines.
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| Assay Principle |
Cell Technology ’s APO-ACTIVE 3 PE Kit
utilizes a rabbit affinity purified polyclonal antibody raised against
amino acid 163-175 of murine caspase 3 (9). This neo epitope is present
on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis
are fixed and permeablized prior to the addition of the antibody. A
secondary PE labeled goat anti rabbit antibody is used to visualize the
bound rabbit anti caspase 3 polyclonal. PE labeling allows for use with
a variety of applications.
|
| Citations |
The endoplasmic reticulum is the site of cholestrol-induced
cytotoxicity in macrophages - Feng, Yao, Li, Devlin et al - Nature
Cell Biology Vol 5, No 9, September 2003, pp 781-792
Deficiency of the Cyclin Kinase inhibitor p21(WAF-1/CIP-1) Promotes
Apoptosis of Activated/Memory T Cells, and inhibits spontaneous
systemic Autoimmunity - J. Exp Medicine; Vol 199, Number 4, Feb 16
2004; 547-557
Melanin
acts as a potent UVB photosensitizer to cause an atypical mode of cell
death in murine skin - Seiji Takeuchi, Wengeng Zhang, Kazumasa
Wakamatsu, Shosuke Ito,Vincent J. Hearing, Kenneth H.
Kraemer and Douglas E. Brash - PNAS U S A. 2004 October 19;
101(42): 15076–15081
Maternal
Antibody Responses to the 52-kd SSA/Ro p200 Peptide and the Development
of Fetal Conduction defects - Clancy, Buyon, Ikeda, et.al -
Arthritis and Rheumatism Vol 52, No 10, October 2005, pp 3079-3086
|
| References |
-
Slee, E. A., C.
Adrain, and S. J. Martin. 1999. Serial Killers: orderingcaspase
activation events in apoptosis. Cell Death and Differ. 6:1067-1074.
-
Walker, N. P., R. V.
Talanian, K. D. Brady, L. C. Dang, N. J. Bump, C. R.Ferenz, S.
Franklin, T. Ghayur, M. C. Hackett and L. D. Hammill. 1994. Crystal
Structure of the Cysteine Protease Interleukin-1ß-Converting
Enzyme: A(p20/p10)2
Homodimer. Cell 78:343-352.
-
Wilson, K. P., J. F.
Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M. A.Navia, M. A.
Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D.
J.Livingston. 1994. Structure and mechanism of interleukin-1 beta
converting enzyme. Nature 370: 270-275.
-
Rotonda, J., D. W.
Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M. Labelle,E. P.
Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A. Thornberry
and J.W. Becker. 1996. The three-dimensional structure of
apopain/CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3(7):
619-625.
-
Kumar, S. 1999.
Mechanisms mediating caspase activation in cell death. Cell Death and
Differ. 6: 1060-1066.
-
Alnemri, E.S. et al
(1996) Cell 87:171
-
Trends Biochem Sci
22,388 (1997)
-
Nature 376, 37 (1995).
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| Kit contents |
|
|
| The
following kits are available: |
Product
|
Catalog No.
|
Size
(No. of Tests) |
Price (US$) |
| APO
ACTIVE 3 PE Antibody Specific Active Caspase 3 Detection Kit |
PAB200-1 |
25 |
$145 |
PAB200-2
|
100 |
$425 |
|
| * Please call 888 7 ASSAYS
(888-727-7297) or email info@celltechnology.com
for volume pricing |
|
|
