 |
| Caspase
/ Apoptosis Detection |
 |
High
Throughput Screening
- Apo3HTS |
|
Caspase
Poly,1,2,3,7,8,9,10 Detection - FAM-FMK |
|
Caspase
Poly, 1,2,3,8,9,10, Detection
- (SR-FMK) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (PE) |
|
| NOS/ROS
Detection Kits |
|
|
| Fluorescent
Enzymatic Assays |
|
|
| Mitochondria
Membrane Potential detection |
|
|
| aCella
- Bioluminescence Assays Kits |
|
|
| Cytotoxicity |
|
|
| ELISA
Products |
|
|
| Cathepsin
B,K,L Detection Kits |
|
|
|
|
|
| |
APO
ACTIVE 3 Phycoerythrin (PE) ™
Antibody Specific Active
Caspase 3 Detection Kit
|
| Key Benefits: |
-
Highly specific for active human and murine caspase
3. Other assays require the utilization of peptide
based (DEVD) reagents that tend to cross-react with
caspase 7 and other caspases.
-
Applications - Works with a flow cytometer,
flourescence microscope, or 96 well plate reader.
-
Yields both quantitative and qualitative results.
Gives a strong positive signal.
-
Can be used in
conjunction with other antibodies or stains.
-
No
need to make cell lysates or run western blots.
Cells can be fixed and analyzed later.
-
Works with
human, mouse and rat cell lines.
|
| Assay Principle |
Cell Technology ’s
APO-ACTIVE 3 PE Kit utilizes a rabbit affinity purified
polyclonal antibody raised against amino acid 163-175
of murine caspase 3 (9). This neo epitope is present
on the p18 subunit of cleaved caspase 3 (9). Cells
undergoing apoptosis are fixed and permeablized prior
to the addition of the antibody. A secondary PE labeled
goat anti rabbit antibody is used to visualize the
bound rabbit anti caspase 3 polyclonal. PE labeling
allows for use with a variety of applications.
|
| Citations |
The endoplasmic reticulum is the site of cholestrol-induced
cytotoxicity in macrophages - Feng, Yao, Li, Devlin et
al - Nature Cell Biology Vol 5, No 9, September
2003, pp 781-792
Deficiency of the Cyclin Kinase inhibitor p21(WAF-1/CIP-1) Promotes Apoptosis of Activated/Memory T Cells, and inhibits spontaneous systemic Autoimmunity
- J. Exp Medicine; Vol 199, Number 4, Feb 16 2004;
547-557
Melanin
acts as a potent UVB photosensitizer to cause an
atypical mode of cell death in murine skin - Seiji
Takeuchi, Wengeng Zhang, Kazumasa Wakamatsu, Shosuke
Ito,Vincent J. Hearing, Kenneth H. Kraemer and Douglas
E. Brash - PNAS U S A. 2004 October 19; 101(42):
15076–15081
Maternal
Antibody Responses to the 52-kd SSA/Ro p200 Peptide and
the Development of Fetal Conduction defects - Clancy,
Buyon, Ikeda, et.al - Arthritis and Rheumatism Vol
52, No 10, October 2005, pp 3079-3086
|
| References |
-
Slee, E. A., C. Adrain, and S.
J. Martin. 1999. Serial Killers: orderingcaspase
activation events in apoptosis. Cell Death and Differ.
6:1067-1074.
-
Walker, N. P., R. V. Talanian,
K. D. Brady, L. C. Dang, N. J. Bump, C. R.Ferenz,
S. Franklin,
T. Ghayur, M. C. Hackett and L. D. Hammill. 1994.
Crystal Structure
of the Cysteine Protease Interleukin-1ß-Converting
Enzyme: A(p20/p10)2 Homodimer. Cell 78:343-352.
-
Wilson, K. P., J. F. Black, J. A. Thomson, E.
E. Kim, J. P. Griffith, M. A.Navia, M. A. Murcko, S.
P.
Chambers, R. A. Aldape, S. A. Raybuck, and D. J.Livingston.
1994. Structure and mechanism of interleukin-1
beta converting enzyme. Nature 370: 270-275.
-
Rotonda, J., D. W. Nicholson, K. M. Fazil, M.
Gallant, Y. Gareau, M. Labelle,E. P. Peterson, D. M.
Rasper,
R. Ruel, J. P. Vaillancourt, N. A. Thornberry and
J.W. Becker. 1996. The three-dimensional structure
of apopain/CPP32,
a key mediator of apoptosis. Nature Struct. Biol.
3(7): 619-625.
-
Kumar, S. 1999. Mechanisms mediating caspase activation
in cell death. Cell Death and Differ. 6: 1060-1066.
-
Alnemri, E.S. et al (1996) Cell 87:171
-
Trends Biochem Sci 22,388 (1997)
-
Nature 376, 37 (1995).
|
| Kit contents |
|
|
| The following
kits are available: |
Product
|
Catalog No.
|
Size
(No. of Tests) |
Price (US$) |
| APO ACTIVE 3 PE
Antibody Specific Active Caspase 3 Detection Kit |
PAB200-1 |
25 |
$135 |
PAB200-2
|
100 |
$395 |
|
| * Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for
volume pricing |
|
|
