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| Caspase
/ Apoptosis
Detection |
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High
Throughput Screening
- Apo3HTS |
|
Caspase
Poly,1,2,3,7,8,9,10 Detection - FAM-FMK |
|
Caspase
Poly, 1,2,3,8,9,10, Detection
- (SR-FMK) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (PE) |
|
| NOS/ROS
Detection Kits |
|
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| Fluorescent
Enzymatic Assays |
|
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| Mitochondria
Membrane Potential detection |
|
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| aCella
- Bioluminescence Assays Kits |
|
|
| Cytotoxicity |
|
|
| ELISA
Products |
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| Cathepsin
B,K,L Detection Kits |
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APO
ACTIVE 3 ™
Antibody Specific Active
Caspase 3 Detection Kit
|
| Key Benefits: |
-
Highly specific for active human and murine caspase
3. Other assays require the utilization of peptide
based (DEVD) reagents that tend to cross-react with
caspase 7 and other caspases.
-
Applications - Works with a fluoresence
microscope, 96 well plate reader, or flow cytometer.
-
Yields both quantitative and qualitative results.
Gives a strong positive signal.
-
Can be used in
conjunction with other antibodies or stains.
-
No
need to make cell lysates or run western blots.
Cells can be fixed and analyzed later.
|
| Assay Principle |
Cell Technology ’s
APO-ACTIVE CASPASE 3 Kit utilizes a rabbit affinity
purified polyclonal antibody raised against amino
acid 163-175 of murine caspase 3 (9). This neo epitope
is present on the p18 subunit of cleaved caspase
3 (9). Cells undergoing apoptosis are fixed and permeablized
prior to the addition of the antibody. A secondary
FITC labeled goat anti rabbit antibody is used to
visualize the bound rabbit anti caspase 3 polyclonal.
|
| Citations |
The endoplasmic reticulum is the site of cholestrol-induced
cytotoxicity in macrophages - Feng, Yao, Li, Devlin et
al - Nature Cell Biology Vol 5, No 9, September
2003, pp 781-792
Deficiency of the Cyclin Kinase inhibitor p21(WAF-1/CIP-1) Promotes Apoptosis of Activated/Memory T Cells, and inhibits spontaneous systemic Autoimmunity
- J. Exp Medicine; Vol 199, Number 4, Feb 16 2004;
547-557
Melanin
acts as a potent UVB photosensitizer to cause an
atypical mode of cell death in murine skin - Seiji
Takeuchi, Wengeng Zhang, Kazumasa Wakamatsu, Shosuke
Ito,Vincent J. Hearing, Kenneth H. Kraemer and Douglas
E. Brash - PNAS U S A. 2004 October 19; 101(42):
15076–15081
Enhanced
growth inhibition of squamous cell carcinoma of the head
and neck by combination therapy of paclitaxel and opioid
growth factor - Patricia McLaughlin, Jeffrey
Jaglowski, et al -
International Journal of Oncology 26: 809-816, 2005
Maternal
Antibody Responses to the 52-kd SSA/Ro p200 Peptide and
the Development of Fetal Conduction defects - Clancy,
Buyon, Ikeda, et.al - Arthritis and Rheumatism Vol
52, No 10, October 2005, pp 3079-3086
Combination
chemotherapy with gemcitabine and biotherapy with opioid
growth factor (OGF) enhances the growth inhibition of
pancreatic adenocarcinoma - Ian S. Zagon,
Jeffrey R. Jaglowski, et.al. - Cancer
Chemotherapy and Pharmacology – Vol 56/ No 5, Nov 2005
|
| References |
-
Slee, E. A., C. Adrain, and S.
J. Martin. 1999. Serial Killers: ordering caspase
activation events in apoptosis. Cell Death and Differ.
6:1067-1074.
-
Walker, N. P., R. V. Talanian,
K. D. Brady, L. C. Dang, N. J. Bump, C. R. Ferenz,
S. Franklin,
T. Ghayur, M. C. Hackett and L. D. Hammill. 1994.
Crystal Structure of the Cysteine Protease Interleukin-1ß-Converting
Enzyme: A (p20/p10)2 Homodimer. Cell 78:343-352.
-
Wilson, K. P., J. F. Black, J. A. Thomson, E.
E. Kim, J. P. Griffith, M. A. Navia, M. A. Murcko,
S.
P. Chambers, R. A. Aldape, S. A. Raybuck, and D.
J. Livingston. 1994. Structure and mechanism of interleukin-1
beta converting enzyme. Nature 370: 270-275.
-
Rotonda, J., D. W. Nicholson, K. M. Fazil, M.
Gallant, Y. Gareau, M. Labelle, E. P. Peterson, D.
M. Rasper,
R. Ruel, J. P. Vaillancourt, N. A. Thornberry and
J. W. Becker. 1996. The three-dimensional structure
of
apopain/CPP32, a key mediator of apoptosis. Nature
Struct. Biol. 3(7): 619-625.
-
Kumar, S. 1999. Mechanisms mediating caspase activation
in cell death. Cell Death and Differ. 6: 1060-1066.
-
Alnemri, E.S. et al (1996) Cell 87:171
-
Trends Biochem Sci 22,388 (1997)
-
Nature 376, 37 (1995).
-
Srinivasan A, et al Cell Death and Differentiation
(1998) 5: 1004-1016
|
| Kit contents |
- 1 vial of Rabbit anti cleaved caspase
3 affinity purified polyclonal antibody
- 1 vial of
FITC labeled Goat anti Rabbit affinity purified polyclonal
antibody
- 1 vial of 10X fixative.
|
| The following
kits are available: |
Product
|
Catalog No.
|
Size
(No. of Tests) |
Price (US$) |
| APO ACTIVE 3 Antibody
Specific Active Caspase 3 Detection Kit |
FAB200-1 |
25 |
$135 |
FAB200-2
|
100 |
$395 |
|
| * Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for
volume pricing |
|
|
