| 10 X Wash Buffer A buffer used to rinse ELISA plates between reagent addition steps |
| Improves Assays: |
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| How it Works: |
Cell Technology's
Wash Buffer is an optimal formulation of buffers and
salts which maximizes the adsorption of antibodies and
antigens onto polystyrene plates. During the adsorption
process, the wash buffer stabilizes coated proteins,
allowing for greater binding reactivity with the detection
molecule, thereby enhancing the specific signal. By generating
a higher specific signal, a lower concentration of coat
protein may be needed when this buffer is used, thereby
saving valuable reagents.
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| Directions: |
Simply
dilute the buffer 1:10 (100mL WB to 900mL diH20)
and it's ready to use. To rinse microtiter plates, the
buffer
may be dispensed through a squirt bottle, a plate washer,
or a multi-channel pipette. Be sure to fill the wells
all the way up (400uL for a 96-well plate). We do NOT
recommend submerging the entire plates in a bath as many
of the wells become contaminated, which decreases the
reliability of the assay.
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* Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for volume pricing