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| Caspase
/ Apoptosis
Detection |
 |
High
Throughput Screening
- Apo3HTS |
 |
Caspase
Poly,1,2,3,7,8,9,10 Detection - FAM-FMK |
 |
Caspase
Poly, 1,2,3,8,9,10, Detection
- (SR-FMK) |
 |
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
 |
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (PE) |
|
| NOS/ROS
Detection Kits |
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| Fluorescent
Enzymatic Assays |
|
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| Mitochondria
Membrane Potential detection |
|
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| aCella
- Bioluminescence Assays Kits |
|
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| Cytotoxicity |
|
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| ELISA
Products |
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| Cathepsin
B,K,L Detection Kits |
|
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| |
Fluoro
SSAO™
Fluorescent Semicarbazide-Sensitive Amine Oxidase Detection
Kit
|
| Key Benefits: |
- Non Radioactive
- Can monitor multiple time points
to follow kinetics.
- Monitors Enzymatic Activity
- One-step, no wash assay.
- Adaptable for High Throughput
format
- Enzyme Positive Control included in Kit
- Applications - Fluorescence Plate Reader
|
| Assay Principle |
Semicarbazide-sensitive
amine oxidase (SSAO) is a common name for a widely
distributed enzyme in nature. In man this enzyme is
present in the vascular system and circulates in plasma.
SSAO differ from the monoamine oxidases A and B in
substrate and inhibitor patterns. These enzymes have
been widely studied and their tissue distribution,
molecular properties, substrate specificities and inhibitor
sensitivities are extensively reviewed (2,3).
SSAO exists in two forms: tissue bound and soluble
(plasma SSAO). Tissue bound SSAO acitvity is associated
with blood vessels, mainly in smooth muscle layers,
however it is also associated with spleen, placenta,
bone marrow, kidney, sclera, retina, endothelial
cells, adipocytes, chondrocytes and fibroblasts.
(4,5). Evidence suggests that Plasma SSAO originates
from the cleavage of membrane-bound form. The possible
sources of plasma SSAO are still unclear, but it
has been suggested that it may be derived from liver,
retina, placenta and bone tissue (6,7,8).
SSAO’s functional role has been suggested
to be involved in: apoptosis, atherogenesis, cell
adhesion, leucocyte trafficking, glucose transport
and local production of hydrogen peroxide. Reports
of elevated levels of SSAO have been reported in
congestive heart failure, diabetes mellitus, alzheimer’s
disease and various other inflammatory diseases (1).
Furthermore byproducts of SSAO deamination, such
as formaldehyde and methylglyoxal, have been proposed
to be involved in pathogenesis of cancer, aging and
atherosclerosis.
The Fluoro SSAO detection kit utilizes a non- fluorescent
detection reagent,
to detect H202 released from
the conversion of Benzylamine to Benzaldehyde via
SSAO. Furthermore H202 oxidizes
this detection reagent in a 1:1 stoichiometry to produce a fluorescent
product resorufin. This oxidation is catalyzed by
Peroxidase.
|
| Reaction: |
Benzylamine + O2+ H2O
+ SSAO Benzaldehyde
+ NH3 + H202 |
H202 + Detection reagent (non-fluorescent)+Peroxidase
Resorufin (fluorescent)
|
|
| References |
-
Yayin Tahiri Kasim et.al., Turk
J Biochem; 29 (3);247-254 (2004); Semicarbazide-Sensitive
Amine Oxidase: Biochemical and Physiological Properties
-
B.A.Callingham,A.Holt,J.Elliott.J.Neural
Transm.(Suppl.)32 ,279 –290 (1990).
-
B.A.Callingham and M.A.Barrand.J.Neural
Transm.(Suppl.)23 ,37 –54 (1987).
-
G.A.Lyles.J.Neural Transm.(Suppl.)41
,387 –396
(1994).
-
K.Magyar, Z.Mészáros and P.Mátyus
. Semicarbazide-sensitive amine oxidase.Its physiological
significance Pure Appl.Chem.,Vol.73,No.9,pp.1393 –1400,2001.
-
Boomsma F.,van Dijk J.,Bhaggoe U.M.,Bouhuizen
A.M.B.,van den Meiracker A.H.(2000)Variation in semicarbazide-sensitive
amine oxidase activity in plasma and tissues of
mammals.Comp.Biochem.
-
Ekblom J.,Gronval J.L.,Garpenstrand H.,Nillson
S.,Oreland L.(2000)Is semicarbazide-sensitive amine
oxidase in blood plasma partly derived from the
skeleton?.Neurobiology,8,129-135.
-
McGuirl M.A., Dooley D.M.(1999)Copper-containing
oxidases. Curr.Opin.Chem.Biol.,3,138-144.
-
Methods Enzymology 142, 617 (1997).
-
Holt A. ; Sharman D.F. ; Baker G.B. ; Palcic M.M.
A Continuous Spectrophotometric Assay for Monoamine
Oxidase and Related Enzymes in Tissue Homogenates
Analytical
Biochemistry, January 1997, vol. 244, no.
2, pp. 384-392(9).
-
Biochem Pharmacol 18, 1447 (1969).
|
| Kit contents
(for 500 assays) |
Description
|
Part#
|
Storage after opening
Kit or Reconstitution of Reagents. |
1 Bottle: 5X Reaction Buffer pH
7.4
|
3019 |
4-80C |
| 1 vial: Detection reagent for 500
Assays |
4008
|
Aliquot in single use vials: Below –200C |
| 1 vial: Horseradish Peroxidase |
6005 |
4-80C |
1 vial: SSAO substrate Benzylamine
|
7001 |
Aliquot in vials: Below –200C |
| 1 vial: SSAO Enzyme |
6006 |
Aliquot in single use vials: Below –200C |
| 1 vial Pargyline: Monoamine Oxidase
B inhibitor (9-11) |
7003 |
Aliquot in vials: Below –200C |
| 1 vial Semicarbazide: Semicarbazide-sensitive
amine oxidase inhibitor. |
7004 |
Aliquot in single use vials: Below –200C |
| The following
kits are available: |
Catalog #
|
Size
|
Price (US$) |
SSAO 100-3
|
500 Tests |
$295 |
|
| * Please call 888 7 ASSAYS (888-727-7297) or
email info@celltechnology.com for volume pricing |
|
|