Hypochlorite Detection Kit™
Fluorescence Ocl (Hypochlorite) Detection Kit |
| Key Benefits: |
- Quenched Cell permeable dye.
- One Step, No wash Homogenous assay.
- Adaptable to High throughput assay platforms.
- Can monitor multiple time points to follow real time kinetics.
- Non-destructive cell based assay allows monitoring of additional parameters.
- Applications - Fluorescence Plate Reader / Flow cytometry / Fluorescence Microscopy
|
| Introduction: |
The two new novel probes, Aminophenyl fluorescein (APF) and Hydroxyphenyl fluorescein (HPF) developed by Tetsuo Nagano et. al. (1), are selective for the detection of highly reactive oxygen species (hROS). Both probes have little reactivity towards other ROS such as: singlet oxygen (021), superoxide (O2-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1. HPF/APF are cell permeable and can be used in combination to detect hypochlorite (-OCl) production in cells (see fig 1). Hypochlorite can be detected by loading two samples, one with APF and the other with HPF. Hypochlorite production is visualized by increase in fluorescence of APF loaded cells and no increase in fluorescence in HPF loaded cells. |
| Assay Principle: |
|
Fig 1. : Detection of Hypochlorite ( -OCl ) in neutrophils. Neutrophils were isolated from porcine blood, washed in Krebs-Ringers phosphate buffer (as described in step V 1. above) and seeded in glass bottom dishes. The cells were then loaded with APF or HPF (10mM final) by incubation for 30 minutes at room temperature. The Dye-loaded neutrophils were stimulated with PMA (2ng/mL). Fluorescence images were acquired before and 10 minutes after stimulation. Excitation: 488nm emission: 505-550 nm barrier filters1. Hypochlorite production can be detected with increase APF fluorescence and no increase in HPF fluorescence.
|
| Reactivity: |
| Reactivity Profile
of APF/HPF: |
ROS (RFU)
|
HPF
(RFU)
Ex:499 Em:515 |
APF
(RFU)
Ex:499 Em:515
|
DCFH-DA
(RFU)
Ex:500 Em:520
|
Hydroxyl Radical: •OH
|
730 |
1200 |
7400 |
Peroxynitrite: ONOO-
|
120
|
560
|
6600 |
| Hypochlorite: -OCl |
6 |
3600 |
86 |
| Oxygen Radical: 1O2 |
5 |
9 |
26 |
| Superoxide: O2-• |
8 |
6 |
67 |
Hydrogen Peroxide : H202
|
2 |
1 |
190 |
| Nitric Oxide: NO |
6 |
1 |
150 |
| Alkylperoxyl Radical: ROO. |
17 |
2 |
710 |
| Autoxidation |
1 |
1 |
2000 |
|
| References: |
- Ken-ichi Setsukinai, Yasuteru Urano, Katsuko Kakinuma, Hideyuki J. Majima , and Tetsuo Nagano. Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 278, No. 5, Issue of January 31, pp. 3170–3175, 2003
Manufactured: by Daiichi Pure Chemicals Co. Ltd. Japan
|
Not for Sale in Japan
*Test size will depend on dilution and volume used per test. For example:
|
| Dilution of APF/HPF |
Volume of test |
Numer of tests |
| 1:500 (10mM final) |
0.2 mL |
147 |
| 1: 1000 (5mM final) |
0.2mL |
294 |
|
