 |
| Caspase
/ Apoptosis
Detection |
 |
High
Throughput Screening
- Apo3HTS |
|
Caspase
Poly,1,2,3,7,8,9,10 Detection - FAM-FMK |
|
Caspase
Poly, 1,2,3,8,9,10, Detection
- (SR-FMK) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (PE) |
|
| NOS/ROS
Detection Kits |
|
|
| Fluorescent
Enzymatic Assays |
|
|
| Mitochondria
Membrane Potential detection |
|
|
| aCella
- Bioluminescence Assays Kits |
|
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| Cytotoxicity |
|
|
| ELISA
Products |
|
|
| Cathepsin
B,K,L Detection Kits |
|
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| |
NOS
Detection Kit™
NOS (Nitric Oxide Synthase) Detection Kit |
| Key Benefits: |
- Cell permeable
- No wash homogenous assay.
- Adaptable to High throughput
assay platforms
- Real time detection of NOS activity.
- Applications - Flow Cytometry, Fluorescence
Plate Reader, Fluorescence Microscope
|
| Assay Principle: |
Diaminofluorescein –2
Diacetate (DAF-2DA) is a non-fluorescent cell permeable
reagent that can measure free Nitric Oxide (NO) and
nitric oxide synthase (NOS) activity in living cells
under physiological conditions. Once inside the cell
the diacetate groups on the DFA-2DA reagent arehydrolyzed
by cytosolic esterases thus releasing FAD-2 and sequestering
the reagent inside the cell. Production of nitric oxide
converts the non-fluorescent dye, DAF-2, to its fluorescent
triaole derivative, DAF-2T .
DAF-2T can be observed by: excitation 488nm and
measuring emission at 515nm.
|
| Reactivity: |
| DAF-2DA can be used to detect
NOS activity in cell culture or tissue sections. This
reagent is not species specific and can also be used
to detect NOS activity in plant cells. |
| References |
-
Direct evidence of NO production
in rat hippocampus and cortex using a new fluorescent
indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport
9, 3345 (1998) Abstract
-
Direct evidence of nitric oxide production from
bovine aortic endothelial cells using new fluorescence
indicators: diaminofluoresceins: N. Nakatsubo,
etal.; FEBS Lett. 427, 263 (1998) Abstract
-
Nitric Oxide Ameliorates Hydrophobic Bile Acid-induced
Apoptosis in Isolated Rat Hepatocytes by Non-mitochondrial
Pathways: E. Gumpricht, et al.; J. Biol.Chem. 277,
25823 (2002) Full Text;
http://www.jbc.org/cgi/content/full/277/28/25823#SEC1
-
Development of a fluorescent indicator for nitric
oxide based on the fluoroscein chromophore: H.
Kojima, et al.; Chem. Pharm. Bull. 46, 373 (1998)Abstract
-
Detection and imaging of nitric oxide with novel
fluorescent indicators: diaminofluoresceins: H.
Kojima, et al.; Anal. Chem. 70, 2446 (1998) Abstract
|
| Product Specifications: |
- Formula: C24H18N2O7
- MW: 446.4
- Appearance: Liquid
- Formulation: DAF-2DA is dissolved
in DMSO at approx 5mM.
- Manufactured: by Daiichi Pure
Chemicals Co. Ltd. Japan
Not For
Sale in Japan |
| The following
kits are available: |
Catalog #
|
Size* |
Price |
NOS200-1
|
0.125 mg per vial at 2.22 mg/mL. |
$125 |
| NOS200-2 |
0.25 mg per vial at 2.22 mg/mL.
|
$245 |
|
| * Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for
volume pricing |
|
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