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| Caspase / Apoptosis
Detection |
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High Throughput Screening
- Apo3HTS |
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Caspase Poly,1,2,3,7,8,9,10
Detection - FAM-FMK |
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Caspase Poly, 1,2,3,8,9,10,
Detection
- (SR-FMK) |
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Antibody Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
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Antibody Specific Caspase 3 Detection
- Apo Active 3 (PE) |
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| NOS/ROS Detection Kits |
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| Fluorescent Enzymatic
Assays |
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| Mitochondria
Membrane Potential detection |
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| aCella - Bioluminescence
Assays Kits |
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| Cytotoxicity |
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| ELISA
Products |
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| Cathepsin B,K,L
Detection Kits |
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NOS Detection Kit™
NOS (Nitric Oxide
Synthase) Detection Kit |
| Key Benefits: |
- Cell permeable
- No wash homogenous assay.
- Adaptable to High throughput assay
platforms
- Real time detection of NOS activity.
- Applications - Flow Cytometry,
Fluorescence Plate Reader, Fluorescence Microscope
|
| Assay Principle: |
Diaminofluorescein
–2 Diacetate (DAF-2DA) is a non-fluorescent cell permeable
reagent that can measure free Nitric Oxide (NO) and nitric oxide
synthase (NOS) activity in living cells under physiological conditions.
Once inside the cell the diacetate groups on the DFA-2DA reagent
arehydrolyzed by cytosolic esterases thus releasing FAD-2 and
sequestering the reagent inside the cell. Production of nitric oxide
converts the non-fluorescent dye, DAF-2, to its fluorescent triaole
derivative, DAF-2T .
DAF-2T can be observed by: excitation 488nm
and measuring emission at 515nm.
|
| Reactivity: |
| DAF-2DA can be used to
detect NOS activity in cell culture or tissue sections. This reagent is
not species specific and can also be used to detect NOS activity in
plant cells. |
| References |
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Direct evidence of NO
production in rat hippocampus and cortex using a new fluorescent
indicator: DAF-2 DA: H. Kojima, et al.; Neuroreport 9, 3345 (1998)
Abstract
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Direct evidence of
nitric oxide production from bovine aortic endothelial cells using new
fluorescence indicators: diaminofluoresceins: N. Nakatsubo, etal.; FEBS
Lett. 427, 263 (1998) Abstract
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Nitric Oxide
Ameliorates Hydrophobic Bile Acid-induced Apoptosis in Isolated Rat
Hepatocytes by Non-mitochondrial Pathways: E. Gumpricht, et al.; J.
Biol.Chem. 277, 25823 (2002) Full Text;
http://www.jbc.org/cgi/content/full/277/28/25823#SEC1
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Development of a
fluorescent indicator for nitric oxide based on the fluoroscein
chromophore: H. Kojima, et al.; Chem. Pharm. Bull. 46, 373
(1998)Abstract
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Detection and imaging
of nitric oxide with novel fluorescent indicators: diaminofluoresceins:
H. Kojima, et al.; Anal. Chem. 70, 2446 (1998) Abstract
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| Product
Specifications: |
- Formula: C24H18N2O7
- MW: 446.4
- Appearance: Liquid
- Formulation: DAF-2DA is dissolved in DMSO
at approx 5mM.
- Manufactured: by Daiichi Pure Chemicals
Co. Ltd. Japan
Not For Sale in Japan
|
| The
following kits are available: |
Catalog #
|
Size* |
Price |
NOS200-1
|
0.125 mg per vial at 2.22 mg/mL. |
$145 |
| NOS200-2 |
0.25 mg per vial at 2.22 mg/mL.
|
$290 |
|
| * Please call 888 7 ASSAYS
(888-727-7297) or email info@celltechnology.com
for volume pricing |
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