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| Caspase
/ Apoptosis Detection |
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High
Throughput Screening
- Apo3HTS |
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Caspase
Poly,1,2,3,7,8,9,10 Detection - FAM-FMK |
|
Caspase
Poly, 1,2,3,8,9,10, Detection
- (SR-FMK) |
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Antibody
Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
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Antibody
Specific Caspase 3 Detection
- Apo Active 3 (PE) |
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| NOS/ROS
Detection Kits |
|
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| Fluorescent
Enzymatic Assays |
|
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| Mitochondria
Membrane Potential detection |
|
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| aCella
- Bioluminescence Assays Kits |
|
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| Cytotoxicity |
|
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| ELISA
Products |
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| Cathepsin
B,K,L Detection Kits |
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Mito
Flow™
Mitochondria Membrane Potential detection for Flow Cytometry |
| Key Benefits: |
- Cell Permeable, Easy One Color Assay for Flow
Cytometry
- Can be used with both suspension and monolayer
adherent cell lines.
- 1 Step, No Wash, Adaptable
for High Throughput format
- Compatible with other
antibodies or stains. For example flourescent protein
expression vectors.
- Applications - Flow Cytometer
|
| Assay Principle: |
Cell Technology’s
Mito Flow assay utilizes a cationic dye to visualize mitochondrial membrane potential (5-7).
The Mito Flow reagent is a cell permeable cationic
dye that has a strong fluorescent signal and exhibits
low membrane potential independent (non specific) binding
and toxicity. In healthy cells the Mito Flow reagent
is accumulated by the mitochondria in proportion to
the DeltaPsi (membrane potential). In most cell lines,
accumulation of the Mito Flow reagent in the mitochondria
results in a higher fluorescence intensity. In apoptotic
cells, where the mitochondrial membrane potential is
compromised, the Mito Flow reagent does not get accumulated
in the mitochondria and these cells exhibit a lower
fluorescence signal. |
| References |
-
Desagher, S., Osen-Sand, A., Nichols, A., Eskes,
R., Montessuit, S., Lauper, S., Maundrell, K.,
Antonsson, B., and Martinou, J.C. Bid-induced conformational
change
of Bax is responsible for mitochondrial cytochrome
c release during apoptosis. J. Cell Biol. 144 (5):
891-901 (1999).
-
Narita, M., Shimizu, S., Ito, T., Chittenden,
T., Lutz, R. J., Matsuda, H., and Tsujimoto,
Y. Bax interacts with the permeability transition pore
to
induce permeability transition and cytochrome
c release in isolated mitochondria. Proc. Natl. Acad.
Sci.
USA 95: 14681-14686 (1998).
-
Basanez, G., Nechushtan, A., Drozhinin, O., Chanturiya,
A., Choe, E., Tutt, S., Wood, K. A., Hsu, Y.
T.,Zimmerberg, J., and Youle, R. J. Bax , but not Bcl-XL
decreases
the lifetime of planar phospholipid bilayer membranes
at subnanomolar concentrations. Proc. Natl. Acad.
Sci. USA 96: 5492-5497 (1999).
-
Luo, X., Budihardio, I., Zou, H., Slaughter,
C., and Wang, X. Bid, a Bcl-2 interacting protein,
mediates cytochrome c release from mitochondria
in response to activation of cell surface death receptors.
Cell 94: 481-490 (1998).
-
Ehrenberg B, Montana V, Wei MD, Wuskell JP, Loew
LM. Membrane potential can be determined in individual
cells from the nernstian distribution of cationic
dyes. Biophys J. 1988 May;53(5):785-94.
-
Farkas DL, Wei MD, Febbroriello P, Carson JH,
Loew LM. Simultaneous imaging of cell and mitochondrial
membrane potentials. : Biophys J. 1989 Dec;56(6):1053-69.
Erratum in: Biophys J 1990 Mar;57(3):following
684.
-
Russell C. Scaduto, Jr. and Lee W. Grotyohann.
Measurement of mitochondrial membrane potential
using fluorescent rhodamine derivatives. Biophys J.
1999
Jan;76(1 Pt 1):469-77.
-
Rajagopal A, Pant AC, Simon SM, Chen Y. In vivo
analysis of human multidrug resistance protein
1 (MRP1) activity using transient expression of fluorescently
tagged MRP1. Cancer Res. 2002 Jan 15;62(2):391-6.
|
| Kit contents: |
- Vial of Mito Flow Dye Part#4004
- Bottle of 10X dilution
buffer Part#3004
|
| The following
kits are available: |
Catalog #
|
Size* |
Price |
| Flo200-2 |
100 Tests |
$225 |
| Flo200-3 |
500 Tests |
$425 |
|
| * Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for
volume pricing |
|
|
