 |
| Caspase / Apoptosis
Detection |
 |
High Throughput Screening
- Apo3HTS |
|
Caspase Poly,1,2,3,7,8,9,10
Detection - FAM-FMK |
|
Caspase Poly, 1,2,3,8,9,10,
Detection
- (SR-FMK) |
|
Antibody Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
|
Antibody Specific Caspase 3 Detection
- Apo Active 3 (PE) |
|
| NOS/ROS Detection Kits |
|
|
| Fluorescent Enzymatic
Assays |
|
|
| Mitochondria
Membrane Potential detection |
|
|
| aCella - Bioluminescence
Assays Kits |
|
|
| Cytotoxicity |
|
|
| ELISA
Products |
|
|
| Cathepsin B,K,L
Detection Kits |
|
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|
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| |
Mito Flow™
Mitochondria
Membrane Potential detection for Flow Cytometry |
| Key Benefits: |
- Cell Permeable, Easy One Color Assay for
Flow Cytometry
- Can be used with both suspension and
monolayer adherent cell lines.
- 1 Step, No Wash, Adaptable for High
Throughput format
- Compatible with other antibodies or
stains. For example flourescent protein expression vectors.
- Applications - Flow Cytometer
|
| Assay Principle: |
|
Cell Technology’s
Mito Flow assay utilizes a cationic dye to visualize mitochondrial
membrane potential (5-7). The Mito Flow reagent is a cell permeable
cationic dye that has a strong fluorescent signal and exhibits low
membrane potential independent (non specific) binding and toxicity. In
healthy cells the Mito Flow reagent is accumulated by the mitochondria
in proportion to the DeltaPsi (membrane potential). In most cell lines,
accumulation of the Mito Flow reagent in the mitochondria results in a
higher fluorescence intensity. In apoptotic cells, where the
mitochondrial membrane potential is compromised, the Mito Flow reagent
does not get accumulated in the mitochondria and these cells exhibit a
lower fluorescence signal.
|
| References |
-
Desagher, S.,
Osen-Sand, A., Nichols, A., Eskes, R., Montessuit, S., Lauper, S.,
Maundrell, K., Antonsson, B., and Martinou, J.C. Bid-induced
conformational change of Bax is responsible for mitochondrial
cytochrome c release during apoptosis. J. Cell Biol. 144 (5): 891-901
(1999).
-
Narita, M., Shimizu,
S., Ito, T., Chittenden, T., Lutz, R. J., Matsuda, H., and Tsujimoto,
Y. Bax interacts with the permeability transition pore to induce
permeability transition and cytochrome c release in isolated
mitochondria. Proc. Natl. Acad. Sci. USA 95: 14681-14686 (1998).
-
Basanez, G.,
Nechushtan, A., Drozhinin, O., Chanturiya, A., Choe, E., Tutt, S.,
Wood, K. A., Hsu, Y. T.,Zimmerberg, J., and Youle, R. J. Bax , but not
Bcl-XL decreases the lifetime of planar phospholipid bilayer membranes
at subnanomolar concentrations. Proc. Natl. Acad. Sci. USA 96:
5492-5497 (1999).
-
Luo, X., Budihardio,
I., Zou, H., Slaughter, C., and Wang, X. Bid, a Bcl-2 interacting
protein, mediates cytochrome c release from mitochondria in response to
activation of cell surface death receptors. Cell 94: 481-490 (1998).
-
Ehrenberg B, Montana
V, Wei MD, Wuskell JP, Loew LM. Membrane potential can be determined in
individual cells from the nernstian distribution of cationic dyes.
Biophys J. 1988 May;53(5):785-94.
-
Farkas DL, Wei MD,
Febbroriello P, Carson JH, Loew LM. Simultaneous imaging of cell and
mitochondrial membrane potentials. : Biophys J. 1989 Dec;56(6):1053-69.
Erratum in: Biophys J 1990 Mar;57(3):following 684.
-
Russell C. Scaduto,
Jr. and Lee W. Grotyohann. Measurement of mitochondrial membrane
potential using fluorescent rhodamine derivatives. Biophys J. 1999
Jan;76(1 Pt 1):469-77.
-
Rajagopal A, Pant AC,
Simon SM, Chen Y. In vivo analysis of human multidrug resistance
protein 1 (MRP1) activity using transient expression of fluorescently
tagged MRP1. Cancer Res. 2002 Jan 15;62(2):391-6.
|
| Kit contents: |
- Vial of Mito Flow Dye Part#4004
- Bottle of 10X dilution buffer Part#3004
|
| The
following kits are available: |
Catalog #
|
Size* |
Price |
| Flo200-2 |
100 Tests |
$245 |
| Flo200-3 |
500 Tests |
$475 |
|
| * Please call 888 7 ASSAYS
(888-727-7297) or email info@celltechnology.com
for volume pricing |
|
|
