• Cell Permeable, Easy One Color Assay for Flow Cytometry.
• Can be used with both suspension and monolayer adherent cell lines.
• Adaptable for High Throughput format.
• Compatible with other antibodies or stains. For example flourescent protein expression vectors.
• Applications - Flow Cytometry.

Cell Technology’s Mito Flow assay utilizes a cationic dye to visualize mitochondrial membrane potential (5-7). The Mito Flow reagent is a cell permeable cationic dye that has a strong fluorescent signal and exhibits low membrane potential independent (non specific) binding and toxicity. In healthy cells the Mito Flow reagent is accumulated by the mitochondria in proportion to the DeltaPsi (membrane potential). In most cell lines, accumulation of the Mito Flow reagent in the mitochondria results in a higher fluorescence intensity. In apoptotic cells, where the mitochondrial membrane potential is compromised, the Mito Flow reagent does not get accumulated in the mitochondria and these cells exhibit a lower fluorescence signal.

Protective effects of veskamide, enferamide, becatamide, and oretamide on H2O2-induced apoptosis of PC-12 cells - Jae B Park – Phytomedicine – March 2011 - doi:10.1016/j.phymed.2011.01.025

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• Vial of Mito Flow Dye Part#4004
• Bottle of 10X dilution buffer Part#3004