• Detection of reduced Thiols in cells or tissue extracts
• Sensitive Fluorescent Assay
• Detection of reduced Thiol levels in apoptosis, metabolism and oxidative stress
• detection of reduced thiols in Bacterial, fungal, plant cells or other samples
• Fluorescent 96 well Plate Reader Readout (excitation: 488nm and emission at 515-530 nm)

  Thiol (reduced) + non-fluorescent Dye   ------->         fluorescent analog

  excitation: 488nm and emission at 515-530 nm

1. Gamaley IA and Klyubin IV (1999) Roles of reactive oxygen species: Signaling and regulation of cellular functions. Int Rev Cytol 188:203–238..

2. Nakamura H, Nakamura K and Yodoi J (1997) Redox regulation of cellular activa-tion. Annu Rev Immunol 15:351–369.

3. 2,4-Dinitrobenzenesulfonyl Fluoresceins as Fluorescent Alternatives to Ellman s Reagent in Thiol-Quantification Enzyme Assays*. Hatsuo Maeda,*Hiromi Matsuno,Mai Ushida, Kohei Katayama,Kanako Saeki,and Norio Itoh. Angew.Chem.Int.Ed.2005 ,44 ,2922 –2925

4. Marchetti,P.,et al .,Redox regulation of apoptosis: impact of thiol oxidation status on mitochondrial function.Eur.J.Immunol.,27 ,289-296 (1997).

1. Dye: 1 vial Dried  ..........................................Part# 4021 - Store at +2-8 C
2. Lysis Buffer: 1 Bottle 25 mL..........................Part# 3053 - Store at +2-8 C
3. GSH Standard: 3 vials dried..........................Part# 7015 -  Store at +2-8 C