Fluoro Sarcosine
Fluorescent Sarcosine Detection Kit
Key Benefits:
  • Easy to Use - No Wash Assay.
  • Detection of Sarcosine in cells, serum, tissue extracts..
  • Easy to Use 96-well Fluorescent Plate reader readout.
Introduction to Fluoro Sarcosine

Sarcosine is natural amino acid that is an important intermediate in the metabolism of choline. Sarcosine is an important component of proteins and plays a significant role in metabolic processes of living cells as a source of serine, creatine, purines and glutathione etc. Sarcosine is present in food sources like legumes, eggs, ham, turkey etc. It is used in a variety of industrial applications such as manufacturing of tooth paste and biodegradable surfactants. Sarcosine was recently reported to activate prostate cancer cells and as a possible marker for prostate cancer progression to metastasis (1). It is also used in adjunctive treatment for Schizophrenia (2) and depression (3).

Cell Technology’s Fluoro Sarcosine assay provides a reliable, sensitive fluorimetric method for the quantification of sarcosine in biological samples.
The Fluoro Sarcosine detection kit utilizes a non-fluorescent detection reagent, which is reduced via an enzyme-coupled reaction in the presence of sarcosine. A sarcosine standard curve is generated to quantify sarcosine in the samples.

Reaction

Sarcosine + non-fluorescent detection reagent+ Enzyme Mix -----> fluorescent analog

Excitation: 530-570nm and Emission at 590-600

Fluorescent Sarcosine Detection Kit


Table 1: We conducted spike and recovery experiments to estimate % recovery of sarcosine. Serum was spiked with sarcosine with the concentrations mentioned in the table above. The samples were processed as described in section IX of the protocol.

Fluorescent Sarcosine Detection Kit


Table 2: We conducted spike and recovery experiments to estimate % recovery of sarcosine. Cell lysates were spiked with sarcosine with the concentrations mentioned in the table above. The samples were processed as described in section VIII: Mammalian Cell Preparation.

Fluorescent Sarcosine Detection Kit


Table 3: Serum samples were diluted 1:5 in standard curve diluent. Jurkat and Daudi cells were prepared as described in the protocol. After the final wash cells were adjusted to 1X106 cells/mL in standard curve diluent. Sarcosine was measured as described in the protocol.

Fluorescent Sarcosine Detection Kit


Figure 1. Sarcosine standard curve was generated as described in the protocol. R2= 0.998


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Product Data Sheet
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Protocol
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MSDS
References:
  1. Sreekumar, Arun; Poisson, Laila M.; Rajendiran, Thekkelnaycke M.; Khan, Amjad P.; Cao, Qi; Yu, Jindan; Laxman, Bharathi; Mehra, Rohit et al. (2009). "Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression". Nature 457 (7231): 910. doi:10.1038/nature07762. PMID 19212411.
  2. Lane H, Huang C, Wu P, Liu Y, Chang Y, Lin P, Chen P, Tsai G (2006). "Glycine transporter I inhibitor, N-methylglycine (sarcosine), added to clozapine for the treatment of schizophrenia". Biol Psychiatry 60 (6): 645–9. doi:10.1016/j.biopsych.2006.04.005. PMID 16780811.
  3. http://clinicaltrials.gov/ct2/show/NCT00977353 Clinicaltrials.gov "N-methylglycine (Sarcosine) Treatment for Depression".
Kit contents:
1. Enzyme Mix (Part# 6024): 1 vial. Upon arrival store at -20°C.
2. Standard Curve Diluent (Part# 3056): 40 mL. Upon arrival store at 2-8°C. Ready to use.
3. Sarcosine Detection Reagent (Part# 4023): 1 vial. Upon arrival store at 2-8°C.
4. Sarcosine Standard (Part# 7021): 1 vial. Upon arrival store at 2-8°C.

 ProductCatalog #Size*Price
Fluoro SarcosineSARC100-2100$345.00

* Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for volume pricing