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The Fluoro NAD/NADH
detection kit utilizes a
non-fluorescent detection reagent, which is oxidized in
the presence NADH to produce its fluorescent analog and
NAD. NAD is further converted to NADH via an enzyme
coupled reaction. The enzyme reaction specifically
reacts with NAD/NADH and not with NADP/NADPH.
Reaction:
1. NADH +
non-fluorescent detection reagent+ electron coupler
-----> fluorescent
analog + NAD
2.
NAD + enzyme coupled reaction
-------> NADH (then proceeds to reaction 1).
excitation:
530-570nm and emission at 590-600nm
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