• Highly effective and stable assay for cell proliferation.
• No need to wash out media from cell samples, just add the reagent directly to your experimental samples.
• Works for both cell viability and proliferation assay.
• Plate can be read in 30 minutes to 3 hours.

Cell Technology introduces a Fluoro IndoBlu™ reagent for cell proliferation and viability assay. Fluoro IndoBlu™ is an oxidation/reduction- based reagent that functions as a cell viability indicator by quantitatively measuring the reducing power of living cells. Fluoro IndoBlu™ is a cell-permeable reagent that is blue in color, but non-fluorescent. When added to cells, the IndoBlu™ reagent is modified by the reducing environment of the viable cell, it turns red in color and becomes highly fluorescent. This change can be detected using fluorescence or absorbance measurements. Fluoro IndoBlu™ is detected at the Excitation = 530 nm and Emission = 590 nm/absorbance at 570nm.

Compared to the traditional cell proliferation assay, Cell Technology’s novel in-cell-culture method is simple, fast and sensitive. The entire assay can be performed in a 96-well microtiter plate in 30 min and the best results are achieved in 3 hours.

Measurement of cell proliferation and viability is frequently used in clinical and experimental immunology as means of assessing cell activation in response to diseases, infections and environmental stimulations. Fluoro IndoBlu™ assay can be used for the measurement of cell proliferation in response to antigens, cytokines, growth factors, and mitogens. It can also be used for the analysis of cytotoxic effects of anticancer drugs, drug resistance, cytotoxic pharmaceutical compounds, and other toxic agents.

1. Al-Nasiry, Geusens N, Hassens M, Luyten C, Pijnengorg R (2007) The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells. Hum Reprod 22: 1304.
2. Coban AY (2012) Rapid determination of methicillin resistance among Staphylococcus aureus clinical isolates by colorimetric methods. J Clin Microbiol. 2012 Apr 18.
3. De Fries R, Mitsuhashi M (1995) Quantification of mitogen induced human lymphocyte proliferation: comparison of alamarBLUE TM assay to 3H-Thymidine incorporation assay. J Clin Lab Anal 9: 89.
4. McMillian MK, Li L, Parker JB, Patel L, Zhong Z, Gunnett JW, Powers WJ, Johnson MD (2002) An improved resazurin-based cytotoxicity assay for hepatic cells. Cell Biol Toxicol,18,157.
5. Nateche F, Martin A, Baraka S, Palomino JC, Khaled S, Portaels F (2006) Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers. J Med Microbiol 55: 857.
6. Rolon M, Vega C, Escario JA, Gomez-Barrio A (2006) Development of resazurin microtiter assay for drug sensibility testing of Trypanosoma cruzi epimastigotes. Parasitol. Res, 99: 103.