• Quick 10 minute assay.
• Can monitor multiple time points to follow kinetics.
• Dual mode, can detect H202 or peroxidase activity.
• One-step, no wash assay.
• Adaptable for High Throughput format.
• Non-destructive cell based assay allows monitoring of additional parameters.
• Applications - Fluorescent Plate Reader.

The Fluoro H202 detection kit utilizes a non-fluorescent detection reagent, to detect H202. H202 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.

The detection reagent can be utilized to detect H202 release from cells or enzyme coupled reactions (1-7)

peroxidase H202 + detection reagent (non-fluorescent) ---------> Resorufin (fluorescent) Excitation: 530-571nm Emmission: 590-600nm

Deficiency of the Bax gene attenuates denervation-induced apoptosis - P. M. Siu and S. E. Alway - Apoptosis Journal Vol 11/No 6 June 2006

Manganese chloride stimulates rat microglia to release hydrogen peroxide -Ping Zhang, Angela Hatter and Bin Liu - Toxicology Letters Volume 173, Issue 2, 10 September 2007, Pages 88-100 -doi:10.1016/j.toxlet.2007.06.013

DHCR24 is a hydrogen peroxide scavenger, protecting cells from oxidative-stress-induced apoptosis - Xiuli Lu, Fukushi Kambe, Xia Cao, Yasuko Kozaki, Takahide Kaji, Takehisa Ishii, and Hisao Seo - Endocrinology, doi:10.1210/en.2008-0024

Superoxide dismutase protects against apoptosis and alveolar enlargement induced by ceramide - Irina Petrache, Terry R. Medler, Amy T. Richter, Krzysztof Kamocki, Ugonma Chukwueke, Lijie Zhen, Yuan Gu, Jeremy Adamowicz, Kelly S. Schweitzer,1 Walter C. Hubbard, Evgeny V. Berdyshev, Giuseppe Lungarella, and Rubin M. Tuder – American Journal of Physiology, Lung Physiol, July 2008, Vol 295, No 1 L44-L53

Dual Role of Vitamin C Utilization in NO2-Induced Oxidative Stress in Lung Tissues of Mice - Yingyi Zhang, Chunyan Ma, Yuhuan Xiao, Chang Liu, Qiang Xu, Yan Wang, Xin Xu and Lin Hao – Bulletin of Environmental Contamination and Toxicology Vol 84, Number 6, 662-666 - DOI: 10.1007/s00128-010-0021-1

Cigarette Smoke-Generated Reactive Oxygen Species Impair Adenosine-Mediated Wound Closure in Bronchial Epithelial Cells - DS Allen-Gipson, JC Jarrell, and H Alvarez-Ramirez – American Journal of Respiratory and Critical care medicine - 2009; 179: A2382

Benzyl isothiocyanate-mediated generation of reactive oxygen species causes cell cycle arrest and induces apoptosis via activation of MAPK in human pancreatic cancer cells - Ravi P. Sahu, Ruifen Zhang, Sanjay Batra, Yan Shi and Sanjay K. Srivastava - Carcinogenesis 2009 30(10):1744-1753

Vibration Disrupts Vascular Function in a Model of Metabolic Syndrome - Kristine Krajnak, Stacey Waugh, Claud Johnson, Roger Miller, Meagan Kiedrowski – Industrial Health Vol 47 (2009), No 5, pp 533-542

Inhibition of xanthine oxidase reduces oxidative stress and improves skeletal muscle function in response to electrically stimulated isometric contractions in aged mice - Michael J. Ryan, Janna R. Jackson, Yanlei Hao, Stephen S. Leonard and Stephen E. Alway – Free Radical Biology and Medicine – April 2011, doi:10.1016/j.freeradbiomed.2011.04.002

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1. 5X Reaction Buffer: 20 ml buffer, pH 7.4
2. Detection reagent: One vial for 500 assays.
3. Hydrogen Peroxide: 200µL of a stabilized 3% solution.
4. Horseradish Peroxidase: 18.9 Units of enzyme