 |
| Caspase
/ Apoptosis
Detection |
 |
High
Throughput Screening
- Apo3HTS |
|
Caspase
Poly,1,2,3,7,8,9,10 Detection - FAM-FMK |
|
Caspase
Poly, 1,2,3,8,9,10, Detection
- (SR-FMK) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (PE) |
|
| NOS/ROS
Detection Kits |
|
|
| Fluorescent
Enzymatic Assays |
|
|
| Mitochondria
Membrane Potential detection |
|
|
| aCella
- Bioluminescence Assays Kits |
|
|
| Cytotoxicity |
|
|
| ELISA
Products |
|
|
| Cathepsin
B,K,L Detection Kits |
|
|
|
|
|
| |
Fluoro
Catalase™
Fluorescent
Catalase Detection Kit |
| Key Benefits: |
-
Sensitive Fluorescent Assay
-
Enzyme Positive Control included
in kit.
-
Can monitor multiple time points
to follow kinetics.
- One-step, No Wash assay.
-
Adaptable for High Throughput
format.
-
Monitors enzymatic activity.
-
Applications - Any standard
Fluorescent Plate Reader.
|
| Assay Principle |
Catalase is an
antioxidant enzyme that catalyses the decomposition
of hydrogen peroxide (H 20 2) to water and oxygen. Catalase
is ubiquitously expressed in mammalian and non-mammalian
aerobic cells containing the cytochrome system. The
enzyme has been isolated from various sources, including
bacteria and plant cells (1-3). Catalase activity varies
greatly from tissue to tissue. Highest activity is
seen
in liver and kidney, while lowest activity is seen
in connective tissue (3). In eukarotic cells, catalase
in concentrated in organelles called peroxisomes (4).
The production of hydrogen peroxide in eukaryotic
cells is an end product result of various oxidases
and superoxide dismutase reactions. Accumulation
of
H202 can result in cellular
damage through oxidation of proteins, DNA and lipids
thus resulting in cell
death and mutagenisis (8-11). H202 role
in oxidative stress related diseases have been widely
studied (8,12).
The Fluoro Catalase detection kit is sensitive assay
that utilizes a non - fluorescent detection reagent to detect H202 substrate
left over from the catalase reaction (5-6).
|
| Reaction: |
H 20 2
+ catalase ----------> Water + oxygen
H 20 2 (Left Over) + Detection
reagent (non-fluorescent) + Peroxidase ---------> Resorufin (fluorescent)
Excitation 530-571
Emission 590-600nm
|
| Citations |
|
| References |
-
Deisseroth,
A., and Dounce, A.L. Catalase: Physical and chemical
properties, mechanism of catalysis, and physiological
role Physiol. Rev., 50, 319-375 (1970).
-
Sebastian Mueller, Hans-Dieter
Riedel and Wolfgang Stremmel. Determination of Catalase
Activity at Physiological
Hydrogen Peroxide Concentrations. Analytical
Biochemistry Volume
245, Issue 1 ,1 February 1997,
Pages 55-60.
-
Deisseroth, A., and Dounce, A.L. Catalase: Physical
and chemical properties, mechanism of catalysis, and
physiological role. Physiol. Rev., 50, 319-375 (1970).
-
Marcel Zámocký and
Franz Koller . Understanding the structure and
function of catalases:
clues from molecular evolution and in vitro
mutagenesis. Progress in Biophys. Mol. Biol., 72,
19-66 (1999).
-
Mingjie Zhou, Zhenjun Diwu, Nataliya Panchuk-Voloshina
and Richard P. Haugland. A Stable Nonfluorescent Derivative
of Resorufin for the Fluorometric Determination of
Trace Hydrogen Peroxide: Applications in Detecting
the Activity of Phagocyte NADPH Oxidase and Other
Oxidases. Anal Biochem 253, 162 (1997).
-
J. G. Mohanty, Jonathan S. Jaffe,
Edward S. Schulman and Donald G. Raible. A highly
sensitive fluorescent
micro-assay of H202 release
from activated human leukocytes using a dihydroxyphenoxazine
derivative. J. Immunol
Methods 202, 133 (1997).
-
Tatyana V.Votyakova ,Ian J.Reynolds.
Detection of hydrogen peroxide with Amplex Red:interference
by NADH and reduced glutathione auto-oxidation. Archives
of Biochemistry and Biophysics, 431:138-144 (2004).
-
Jingxiang Bai, Ana M. Rodriguez, J. Andres Melendez,
and Arthur I. Cederbaum. Overexpression of Catalase
in Cytosolic or Mitochondrial Compartment Protects
HepG2 Cells against Oxidative Injury. J. Biol. Chem.,
Sep 1999; 274: 26217 - 26224
-
Tada-Oikawa, S. et al., FEBS Lett.,
442, 65-69 (1999).
-
Hampton, M.B., and Orrenius, S.,
FEBS Lett., 414, 552-556 (1997).
-
Kowaltowski, A.J. et al., FEBS
Lett., 473, 177-182 (2000).
-
Tome, M.E. et al., Cancer Res.,
61, 2766-2733 (2001).
|
| Kit contents
(for 500 assays) |
Description
|
Part#
|
Storage after opening Kit or
Reconstitution of Reagents. |
1 Bottle: 5X Reaction Buffer pH 7.4
|
3021 |
4-80C |
| 1 vial: Detection reagent
|
4010
|
Undiluted : 4-80C
Diluted: Aliquot in single use vials: Below –200C
|
| 1 vial: Horseradish Peroxidase |
6009 |
4-80C undiluted. Diluted: below –200C. |
| 1 vial: Hydrogen Peroxide |
3022 |
4-80C. |
| 1 vial: Catalase enzyme |
6008 |
Crystalline suspension: 4-80C |
| The following
kits are available: |
Catalog #
|
Size
|
Price (US$) |
FLOCAT 100-3
|
500 Tests |
245 |
|
| * Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for
volume pricing |
|
|
24
Reductase Is a Hydrogen Peroxide Scavenger, Protecting Cells from Oxidative
Stress-Induced Apoptosis
