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| Caspase
/ Apoptosis
Detection |
 |
High
Throughput Screening
- Apo3HTS |
|
Caspase
Poly,1,2,3,7,8,9,10 Detection - FAM-FMK |
|
Caspase
Poly, 1,2,3,8,9,10, Detection
- (SR-FMK) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (PE) |
|
| NOS/ROS
Detection Kits |
|
|
| Fluorescent
Enzymatic Assays |
|
|
| Mitochondria
Membrane Potential detection |
|
|
| aCella
- Bioluminescence Assays Kits |
|
|
| Cytotoxicity |
|
|
| ELISA
Products |
|
|
| Cathepsin
B,K,L Detection Kits |
|
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| |
Fluoro
EPO™
Fluorescent Eosinophil
Peroxidase Detection
Kit
|
| Key Benefits: |
- Can monitor multiple time
points to follow kinetics.
- One-step, no wash assay.
- Adaptable for High Throughput
format
- Highly Sensitive
- Applications - Fluorescence (Plate Reader)
or Absorbance
|
| Assay Principle |
Eosinophil
peroxidase (EPO) is the most abundant enzyme found in
eosinophils. It is the major cytotoxic agent released
by activated eosinophils and uses
hydrogen peroxide to generate reactive oxidants from
halides and pseudo halide thiocyanate [1,2]. Eosinophils
peroxidase
has been shown to have antimycobacterial
activity [3], however
it is also implicated in tissue
damage that occurs in asthma and other diseases [4,5].
Currently, the function of eosinophil
involvement in the immune response is being redefined.
Once considered a cell involved in host protection of
parasitic infection, eosinophils multiple functions as
leukocytes involved in the initiation and propagation
of diverse inflammatory responses are being
investigated. Eosinophils are further involved as
modulators of innate and adaptive immunity [6].
|
H2O2
+ Detection reagent (non-fluorescent)+ EPO
--------->
fluorescent
analog
Excitation:530-571nm; Emission: 590-600nm
|
| Applications |
- Detection
of EPO activity in isolated eosinophils.
- Functional
studies of eosinophil degranulation.
- In-vitro
eosinophil chemotaxis studies.
|
| References |
-
Mayeno, A. N., Curran, A. J., Roberts, R. L. and
Foote, C. S. (1989) Eosinophils preferentially use
bromide to generate halogenating agents. J. Biol.
Chem. 264, 5660±5668
.
-
Slungaard, A. and Mahoney, J. R. (1991) Thiocyanate is
the major substrate for
eosinophil
peroxidase in physiologic ¯uids. J. Biol. Chem.
266, 4903±4910 (Pruitt, K. M. and Tenovuo, J. V.,
eds.), pp. 31±53, Marcel Dekker, New York
-
Human
Eosinophil Peroxidase Induces Surface Alteration,
killing, and lysis of Mycobacterium
tuberculosis", Infection and Immunity, Feb.
2003, p. 605-613 by The American Society for
Microbiology.
-
Bousquet,
J., Chanez, P., Lacoste, J. Y., Barneon, G.,
Ghavanian, N., Enander, I., Venge, P., Ahlstedt, S.,
Simony-Lafontaine, J., Godard, P. and Michel, F.
(1990) Eosinophilic in¯ammation in asthma. New
Engl. J. Med. 323, 1033±1039
-
Parrillo,
J. E., Borer, J. S., Henry, W. L., Wolff, S. M. and
Fauci, A. S. (1979) The cardiovascular
manifestations of the hypereosinophilic syndrome.
Prospective study of 26 patients, with review of the
literature. Am. J. Med. 67, 572-582
-
Marc E. Rothenberg and Simon P. Hogan..
THE
EOSINOPHIL Annual
Review of Immunology,
Vol. 24: 147-174 (Volume publication
date April 2006.)
-
Mingjie
Zhou, Zhenjun Diwu, Nataliya Panchuk-Voloshina and
Richard P. Haugland. A Stable Nonfluorescent
Derivative of Resorufin for the Fluorometric
Determination of Trace Hydrogen Peroxide:
Applications in Detecting the Activity of Phagocyte
NADPH Oxidase and Other Oxidases. Anal Biochem 253, 162 (1997).
-
J. G. Mohanty, Jonathan S. Jaffe, Edward S. Schulman
and Donald G. Raible. A highly sensitive fluorescent
micro-assay of H2O2 release
from activated human leukocytes using a
dihydroxyphenoxazine derivative. J.
Immunol Methods 202, 133 (1997).
-
Tatyana V. Votyakova
and Ian J. Reynolds. Membrane Potential dependent and
-independent production of reactive oxygen species
by rat brain mitochondria.
J Neurochem 79, 266 (2001).
-
Chun Song, Abu B. Al-Mehdi, and Aron B. Fisher. An immediate endothelial cell signaling response to lung
ischemia.
Am J Physiol Lung Cell Mol Physiol 281, L993
(2001).
-
Samantha C. Richer and W.C.L. Ford.
A
critical investigation of NADPH oxidase activity in
human spermatozoa.
Mol Hum Reprod 7, 237 (2001).
-
William G. Gutheil, Miglena E. Stefanova and Robert
A. Nicholas. Fluorescent Coupled Enzyme Assays for
 -Alanine:
Application to Penicillin-Binding Protein and
Vancomycin Activity Assays. Anal Biochem 287, 196 (2000).
-
Dominik
Peus, Remus A. Vasa, Astrid Beyerle, Alexander Meves,
Carsten Krautmacher and Mark R. Pittelkow. UVB Activates ERK1/2 and p38 Signaling
Pathways via Reactive Oxygen Species in Cultured
Keratinocytes. J
Invest Dermatol 112, 751 (1999).
-
Tatyana
V.Votyakova ,Ian J.Reynolds. Detection of hydrogen peroxide with Amplex Red:interference
by NADH and reduced glutathione auto-oxidation. Archives
of Biochemistry and Biophysics, 431:
138-144 (2004).
- D.
W. R. Hall, Bridget W. Logan and G. H. Parsons.
Further studies on the inhibition of monoamine
oxidase by M & B 9302 (clorgyline)—I
.Substrate specificity in various mammalian species.
Biochemical Pharma
|
| Kit contents (for 100 assays) |
- 5X Reaction Buffer: 60ml
..........................................Part 3011
- Detection reagent: One vial for 100
assays....................Part
4007
- Hydrogen Peroxide: 1000µL of a stabilized 3%
solution...Part 3012
- Eosinophil Peroxidase: 100µL in 1 vial at 10 units/ml......Part 6016
|
| The following
kits are available: |
Catalog #
|
Size
|
Price (US$) |
FLEPO 100-2
|
100 Tests |
$375 |
|
| * Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for
volume pricing |
|
|
