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| Caspase
/ Apoptosis
Detection |
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High
Throughput Screening
- Apo3HTS |
|
Caspase
Poly,1,2,3,7,8,9,10 Detection - FAM-FMK |
|
Caspase
Poly, 1,2,3,8,9,10, Detection
- (SR-FMK) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (PE) |
|
| NOS/ROS
Detection Kits |
|
|
| Fluorescent
Enzymatic Assays |
|
|
| Mitochondria
Membrane Potential detection |
|
|
| aCella
- Bioluminescence Assays Kits |
|
|
| Cytotoxicity |
|
|
| ELISA
Products |
|
|
| Cathepsin
B,K,L Detection Kits |
|
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|
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| |
| Cathepsin Detect |
|
| Substrate-based
assays to detect protease activity within whole
living cells |
|
| Key Benefits: |
- Cell permeable, allowing for detection in living
cells
- Fluorogenic
- No wash step necessary
|
| Assay Principle: |
Our Cathepsin Detect
products are substrate-based assays designed to detect
protease activity within whole living cells, using a
fluorescence microscope. Cell Technology’s Cathepsin
Detect assays are based on a cresyl violet leaving group
that fluoresces once the enzyme specific peptides are
cleaved. By conjugating the Cathepsin Detect to different
peptide sequences, different enzymes can be detected.
|
| Kit Contents: |
- Cathepsin Detect™ lyophilized reagent
- Hoechst 33342 Stain
- Acridine Orange
|
| References: |
-
Buhling F, Fengler A, Brandt W,
Welte T, Ansorge S, Nagler, DK. Review: novel cysteine
proteases of the papain family. Adv Exp Med Biol
2000; 477:241-254.
- Gerber A, Welte T, Ansorge S, Buhling F. Expression
of cathepsin B and L in human lung epithelial cells
is regulaed by cytokines. Adv Exp Med Biol 2000; 477:287-292.
-
Kas J, Werle B, Lah T, Brunner
N. Cysteine proteinases and their inhibitors in extracellular
fluids: markers for diagnosis and prognosis in cancer.
Int J Biol Markers 2000; 15:84-89.
-
Bank U, Kruger S, Langner J, Roessner
A. Review: peptidases and peptidase inhibitors in
the pathogenesis of diseases. Adv Exp Med Biol 2000;
477:349-378.
-
Guinec N, Fumeron VD, Pagano M.
In vitro study of basement membrane degredation by
the cysteine proteinases, cathepsin B, B-like, and
L. Digestion of collagen IV, laminin, fibronectin,
and release of gelatinase activities from basement
membrane fibronectin. Biol Chem Hoppe-Seyler 1993;
374:1135-1146.
-
Frosch BA, Berquin I, Emmert-Buck
MR, Moin K, Sloane BF. Molecular regulation, membrane
association and secretion of tumor cathepsin B. APMIS
1999; 107:28-37.
-
Bossard MJ, Tomaszek TA, Thompson
SK, Amegadzie BY et al. Proteolytic activity of human
osteoclast cathepsin K. J Biol Chem 1996; 271:12517-12524.
-
Buhling F, Waldburg N, Gerber
A, Hackel C, Kruger S, Reinhold D, Bromme D, Weber
E, Ansorge S, Welte T. Cathepsin K expression in
human lung. Adv Exp Med Biol 2000; 477: 281-286.
-
Leung-Toung R, Li W, Tam TF, Kariman
K. Thiol-dependent enzymes and their inhibitors:
a review. Curr Med Chem 2002; 9:979-1002.
-
1Turk B, Turk D, Turk V. Lysosomal
cysteine proteases : more than scavengers. Biochim
Biophys Acta 2000; 1477:98-111.
-
Van Noorden CJF, Boonacker E,
Bissell ER, Meijer AJ, Van Marle J, Smith RE. Ala-Pro-cresyl
violet, a synthetic fluorogenic substrate for the
analysis of kinetic parameters of dipeptidyl peptidase
IV (CD26) in individual living rat hepatocytes. Anal
Biochem 1997; 252:71-77.
-
Boonacker E, Van Noorden CJF.
Enzyme cytochemical techniques for metabolic mapping
in living cells, with special reference to proteolysis.
J Histochem Cytochem 2001; 49:1473-1486.
-
Traganos F, Darzynkiewicz Z. Lysosomal
proton pump activity: supravital cell staining with
staining with acridine orange differentiates leukocyte
subpopulations. Methods Cell Biol 1994; 41:185-194.
|
| Cathepsin
Detect Assay Kits |
Catalog Number
|
Description
|
Size |
Price |
| CDK100-1 |
Cathepsin Detect Cathepsin B Assay Kit |
25 test |
$175 |
| CDK100-2 |
Cathepsin Detect Cathepsin B Assay Kit |
100 test |
$425 |
| CDK200-1 |
Cathepsin Detect Cathepsin K Assay Kit |
25 test |
$175 |
| CDK200-2 |
Cathepsin Detect Cathepsin K Assay Kit |
100 test |
$425 |
| CDK300-1 |
Cathepsin Detect Cathepsin L Assay Kit |
25 test |
$175 |
| CDK300-2 |
Cathepsin Detect Cathepsin L Assay Kit |
100 test |
$425 |
|
| * Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for
volume pricing |
|
|
