 |
| Caspase /
Apoptosis Detection |
 |
High Throughput Screening
- Apo3HTS |
|
Caspase
Poly,1,2,3,7,8,9,10 Detection -
FAM-FMK |
|
Caspase Poly,
1,2,3,8,9,10, Detection
- (SR-FMK) |
|
Antibody Specific Caspase 3
Detection
- Apo Active 3 (FITC) |
|
Antibody Specific Caspase 3
Detection
- Apo Active 3 (PE) |
|
| NOS/ROS
Detection Kits |
|
|
| Fluorescent
Enzymatic Assays |
|
|
| Mitochondria
Membrane Potential detection |
|
|
| aCella -
Bioluminescence Assays Kits |
|
|
| Cytotoxicity |
|
|
| ELISA
Products |
|
|
| Cathepsin B,K,L
Detection Kits |
|
|
|
|
|
| |
|
Apo 3/7
HTS™
High Throughput
Screen for Caspase
3/7 detection
|
| Key
Benefits: |
-
Homogenous assay for
active caspase
3/7.
-
Breakthrough in cell
lysis buffer and
preservation of caspase activity.
-
Results in a No wash
one-step assay
-
No need to wash out
media from cell
samples, just add the reagent directly to your experimental samples.
-
Easy to Use: No need
to make cell
lysates or run western blots.
-
Works with suspension
and adherent
cells.
-
Applications
- Standard Fluorescence Plate
Reader
|
| Assay
Principle |
Cell
Technology’s Apo 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110
peptide substrate for caspase 3/7 detection. The absorption and
emission properties of the R110 dye are suppressed when attached to the
z-DEVD peptide sequence. When R110 is cleaved away, by active
caspase3/7, form the quenching DEVD sequence, the free dye excites at
488nm and emits at 515-530 nm.. As a result of a novel and proprietary
Lysis Buffer System, the Apo 3/7 HTS Assay is a homogenous platform
that can be utilized for high throughput fluorescence plate reader
applications. The reagent is directly added to the samples thus
eliminating any wash steps.
|
| Citations
|
|
|
| References |
-
Slee, E. A., C.
Adrain, and S. J.
Martin. 1999. Serial Killers: ordering caspase activation events in
apoptosis. Cell Death and Differ. 6:1067-1074.
-
Walker, N. P., R. V.
Talanian, K. D.
Brady, L. C. Dang, N. J. Bump, C. R.Ferenz, S. Franklin, T. Ghayur, M.
C. Hackett and L. D. Hammill. 1994. Crystal Structure of the Cysteine
Protease Interleukin-1ß-Converting Enzyme: A (p20/p10)2
Homodimer. Cell 78:343-352.
-
Wilson, K. P., J. F.
Black, J. A.
Thomson, E. E. Kim, J. P. Griffith, M. A.Navia, M. A. Murcko, S. P.
Chambers, R. A. Aldape, S. A. Raybuck, and D. J.Livingston. 1994.
Structure and mechanism of interleukin-1 beta converting enzyme. Nature
370: 270-275.
-
Rotonda, J., D. W.
Nicholson, K. M.
Fazil, M. Gallant, Y. Gareau, M. Labelle,E. P. Peterson, D. M. Rasper,
R. Ruel, J. P. Vaillancourt, N. A. Thornberry and J.W. Becker. 1996.
The three-dimensional structure of apopain/CPP32, a key mediator of
apoptosis. Nature Struct. Biol. 3(7): 619-625.
-
Kumar, S. 1999.
Mechanisms mediating
caspase activation in cell death. Cell Death and Differ. 6: 1060-1066.
-
Alnemri, E.S. et al
(1996) Cell 87:171
-
Trends Biochem Sci
22,388 (1997)
|
| Kit
contents |
- 1
vial Caspase 3/7 Reagent. (z-DEVD)2
Rhodamine 110 Part#4004
- 1
Bottle of Cell Lysis Buffer
|
| The
following kits are
available: |
Catalog
#
|
Size
|
Price
(US$) |
APO200-2
|
100
Tests |
$275 |
| APO200-3 |
500
Tests
|
$745 |
APO200-4
|
1000
Tests
|
$1395 |
|
| * Please call
888 7 ASSAYS (888-727-7297)
or email info@celltechnology.com
for volume pricing |
|
|
