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| Caspase
/ Apoptosis
Detection |
 |
High
Throughput Screening
- Apo3HTS |
|
Caspase
Poly,1,2,3,7,8,9,10 Detection - FAM-FMK |
|
Caspase
Poly, 1,2,3,8,9,10, Detection
- (SR-FMK) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (FITC) |
|
Antibody
Specific Caspase 3 Detection
- Apo Active 3 (PE) |
|
| NOS/ROS
Detection Kits |
|
|
| Fluorescent
Enzymatic Assays |
|
|
| Mitochondria
Membrane Potential detection |
|
|
| aCella
- Bioluminescence Assays Kits |
|
|
| Cytotoxicity |
|
|
| ELISA
Products |
|
|
| Cathepsin
B,K,L Detection Kits |
|
|
|
|
|
| |
Apo
3/7 HTS™
High Throughput Screen for Caspase 3/7 detection
|
| Key Benefits: |
-
Homogenous assay for active caspase 3/7.
-
Breakthrough
in cell lysis buffer and preservation of caspase
activity.
-
Results in a No wash one-step assay
-
No need to
wash out media from cell samples, just add the
reagent directly to your experimental
samples.
-
Easy to Use: No need to make cell
lysates or run western blots.
-
Works with suspension
and adherent cells.
-
Applications - Standard
Fluorescence Plate Reader
|
| Assay Principle |
Cell Technology’s
Apo 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110
peptide substrate for caspase 3/7 detection. The
absorption and emission properties of the R110 dye
are suppressed when attached to the z-DEVD peptide
sequence. When R110 is cleaved away, by active caspase3/7,
form the quenching DEVD sequence, the free dye excites
at 488nm and emits at 515-530 nm.. As a result of
a novel and proprietary Lysis Buffer System, the
Apo 3/7 HTS Assay is a homogenous platform that can
be utilized for high throughput fluorescence plate
reader applications. The reagent is directly added
to the samples thus eliminating any wash steps.
|
| Citations |
|
| References |
-
Slee, E. A., C. Adrain, and S.
J. Martin. 1999. Serial Killers: ordering caspase
activation events in apoptosis. Cell Death and Differ.
6:1067-1074.
-
Walker, N. P., R. V. Talanian,
K. D. Brady, L. C. Dang, N. J. Bump, C. R.Ferenz,
S. Franklin,
T. Ghayur, M. C. Hackett and L. D. Hammill. 1994.
Crystal Structure
of the Cysteine Protease Interleukin-1ß-Converting
Enzyme: A (p20/p10)2 Homodimer. Cell 78:343-352.
-
Wilson, K. P., J. F. Black, J. A. Thomson, E.
E. Kim, J. P. Griffith, M. A.Navia, M. A. Murcko, S.
P.
Chambers, R. A. Aldape, S. A. Raybuck, and D. J.Livingston.
1994. Structure and mechanism of interleukin-1
beta converting enzyme. Nature 370: 270-275.
-
Rotonda, J., D. W. Nicholson, K. M. Fazil, M.
Gallant, Y. Gareau, M. Labelle,E. P. Peterson, D. M.
Rasper,
R. Ruel, J. P. Vaillancourt, N. A. Thornberry and
J.W. Becker. 1996. The three-dimensional structure
of apopain/CPP32,
a key mediator of apoptosis. Nature Struct. Biol.
3(7): 619-625.
-
Kumar, S. 1999. Mechanisms mediating caspase activation
in cell death. Cell Death and Differ. 6: 1060-1066.
-
Alnemri, E.S. et al (1996) Cell 87:171
-
Trends Biochem Sci 22,388 (1997)
|
| Kit contents |
- 1 vial Caspase 3/7 Reagent. (z-DEVD)2 Rhodamine 110 Part#4004
- 1 Bottle of Cell Lysis Buffer
|
| The following
kits are available: |
Catalog #
|
Size
|
Price (US$) |
APO200-2
|
100 Tests |
$275 |
| APO200-3 |
500 Tests
|
$745 |
APO200-4
|
1000 Tests
|
$1395 |
|
| * Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for
volume pricing |
|
|
