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Caspase / Apoptosis Detection
High Throughput Screening
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Apo 3/7 HTS™
High Throughput Screen for Caspase 3/7 detection

Key Benefits:
  • Homogenous assay for active caspase 3/7.
  • Breakthrough in cell lysis buffer and preservation of caspase activity.
  • Results in a No wash one-step assay
  • No need to wash out media from cell samples, just add the reagent directly to your experimental samples.
  • Easy to Use: No need to make cell lysates or run western blots.
  • Works with suspension and adherent cells.
  • Applications - Standard Fluorescence Plate Reader 
Assay Principle

Cell Technology’s Apo 3/7 HTS Assay utilizes the quenched (z-DEVD)2-R110 peptide substrate for caspase 3/7 detection. The absorption and emission properties of the R110 dye are suppressed when attached to the z-DEVD peptide sequence. When R110 is cleaved away, by active caspase3/7, form the quenching DEVD sequence, the free dye excites at 488nm and emits at 515-530 nm.. As a result of a novel and proprietary Lysis Buffer System, the Apo 3/7 HTS Assay is a homogenous platform that can be utilized for high throughput fluorescence plate reader applications. The reagent is directly added to the samples thus eliminating any wash steps.

Citations
 
References
  1. Slee, E. A., C. Adrain, and S. J. Martin. 1999. Serial Killers: ordering caspase activation events in apoptosis. Cell Death and Differ. 6:1067-1074.
  2. Walker, N. P., R. V. Talanian, K. D. Brady, L. C. Dang, N. J. Bump, C. R.Ferenz, S. Franklin, T. Ghayur, M. C. Hackett and L. D. Hammill. 1994. Crystal Structure of the Cysteine Protease Interleukin-1ß-Converting Enzyme: A (p20/p10)2 Homodimer. Cell 78:343-352.
  3. Wilson, K. P., J. F. Black, J. A. Thomson, E. E. Kim, J. P. Griffith, M. A.Navia, M. A. Murcko, S. P. Chambers, R. A. Aldape, S. A. Raybuck, and D. J.Livingston. 1994. Structure and mechanism of interleukin-1 beta converting enzyme. Nature 370: 270-275.
  4. Rotonda, J., D. W. Nicholson, K. M. Fazil, M. Gallant, Y. Gareau, M. Labelle,E. P. Peterson, D. M. Rasper, R. Ruel, J. P. Vaillancourt, N. A. Thornberry and J.W. Becker. 1996. The three-dimensional structure of apopain/CPP32, a key mediator of apoptosis. Nature Struct. Biol. 3(7): 619-625.
  5. Kumar, S. 1999. Mechanisms mediating caspase activation in cell death. Cell Death and Differ. 6: 1060-1066.
  6. Alnemri, E.S. et al (1996) Cell 87:171
  7. Trends Biochem Sci 22,388 (1997)
Kit contents
  • 1 vial Caspase 3/7 Reagent. (z-DEVD)2 Rhodamine 110 Part#4004
  • 1 Bottle of Cell Lysis Buffer
The following kits are available:
Catalog #
Size
Price (US$)
APO200-2
100 Tests $275
APO200-3 500 Tests
$745
APO200-4
1000 Tests
$1395
* Please call 888 7 ASSAYS (888-727-7297) or email info@celltechnology.com for volume pricing
 
 Product Data Sheet
 Protocol
 MSDS