A new novel probe,
Aminophenyl fluorescein (APF), developed by Tetsuo
Nagano et. al. (1), is a general selective indcator for
the detection of highly Reactive Oxygen Species (hROS).
The
probe has little reactivity towards other ROS such as:
singlet oxygen (O21), superoxide
(O2 ), hydrogen peroxide
(H2O2), nitric oxide (NO),
and alkyl peroxide (RO2) (see
table below)1.
APF is a cell permeable indicator that can be
used to detect Hydroxyl Radical (.OH),
Peroxynitrite: (ONOO- ) and
hypochlorite (-OCl) production in cells.
Several
fluorescent probes such as
2,7-dichlorodihydrofluorescein (DCFH) and
di-hydrorhodamine123,
have been developed to detect Reactive Oxygen Species
(ROS). As Hempel and co-workers (2) have indicated,
DCFH and di-hydrorhodamine123
can react with various ROS and oxidizing species such
as hydrogen peroxide, hypochlorite, superoxide, nitric
oxide, ferrous ion, and others. These probes should be
considered as detecting a broad range of ROS (2).
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